Injection drug use as a mediator between client-perpetrated abuse and HIV status among female sex workers in two Mexico-US border cities.

We examined relationships between client-perpetrated emotional, physical, and sexual abuse, injection drug use, and HIV-serostatus among 924 female sex workers (FSWs) in Tijuana and Ciudad Juarez, two large Mexico-US border cities. We hypothesized that FSWs' injection drug use would mediate the relationship between client-perpetrated abuse and HIV-seropositivity. The prevalence of client-perpetrated emotional, physical, and sexual abuse in the past 6 months was 26, 18, and 10% respectively; prevalence of current injection drug use and HIV was 12 and 6%, respectively. Logistic regression analyses revealed that client-perpetrated sexual abuse was significantly associated with HIV-seropositivity and injection drug use, and that injection drug use was positively associated with HIV-seropositivity. Injection drug use partially mediated the relationship between client-perpetrated sexual abuse and HIV-seropositivity. Results suggest the need to address client-perpetrated violence and injection drug use when assessing HIV risk among FSWs.

Introduction

This study examined the relationship between emotional, physical, and sexual abuse perpetrated by clients, injection drug use, and HIV-seropositivity among female sex workers (FSWs) in Tijuana and Ciudad (Cd.) Juarez, two large Mexico-US border cities where sex work is legally permitted within specific districts (usually referred to as the “Zona Roja” or red light district). Singer [1] has identified the co-occurrence of substance abuse, violence, and AIDS as the SAVA syndemic: mutually reinforcing connections between these conditions that disproportionately affect US inner-city populations living in poverty. Syndemics theory posits that endemic and epidemic conditions such as violence, substance abuse and AIDS in disadvantaged populations occur as a result of broader political, economic, and social factors [2]. Like the SAVA syndemic in the US, FSWs in Tijuana and Cd. Juarez are experiencing disproportionate rates of violence, substance abuse, and HIV/AIDS compared to the larger Mexican population [3-5]. Tijuana and Cd. Juarez are economically depressed cities situated on major drug trafficking routes [6], making this region a nexus for violence, drug abuse, prostitution, sexual tourism, and rising rates of HIV and STIs.

HIV prevalence among FSWs in Tijuana and Cd. Juarez is significantly higher than that of the general Mexican adult population. HIV prevalence among FSWs in Tijuana was estimated to be 6% in 2005, with FSWs who inject drugs (FSW-IDUs) showing an even higher prevalence of 14% [7]. The national rate of HIV prevalence for Mexican adults is 0.3% [3]. In addition, the majority of FSWs in Tijuana and Cd. Juarez are low-income and report entering sex work out of economic necessity to support their family [8]. Thus, SAVA syndemic theory may provide a better understanding of the contextual factors that contribute to HIV risk among FSWs in the US-Mexico border region.

As suggested by Amaro over a decade ago [9], several studies have examined intimate partner violence (IPV) and history of abuse in relation to heterosexual women’s HIV risk. Although an empirical understanding of the relationship between current experiences of violence and HIV infection is still emerging, Maman et al. [10] reviewed several theories that may explain why violence victimization predisposes women to contracting HIV: (1) through forced or coercive sexual intercourse with an HIV-infected partner; (2) by limiting women’s ability to negotiate safe sexual behaviors; and (3) by establishing a pattern of sexual risk-taking among individuals assaulted in childhood and adolescence. More recently, studies have documented the association between IPV and HIV among heterosexual women in different international settings [11, 12] and age groups [13]. Specific risk behaviors associated with IPV include inconsistent condom use and increased likelihood of engaging in sex with an HIV-infected partner or an injection drug user (IDU) [14].

Ethnographic studies have documented the prevalence of history of abuse (i.e., history of childhood abuse, IPV, and client-perpetrated violence) and of HIV infection among FSWs in various international settings [15-17]. History of violence among FSWs has been associated with increased drug use and greater HIV vulnerability. For example, FSWs in India [18] who reported abuse from clients and intimate partners were less likely to use condoms and were therefore at increased sexual risk for HIV. In a study of FSWs in Canada, increased violence by clients and sex and drug-related HIV risk behaviors were associated with sharing illicit drugs with clients [16]. Surratt [19] reported that among FSWs in the US Virgin Islands, those who used illicit drugs were significantly more likely to report unprotected sex, client violence, and STIs. FSWs in New York City who injected drugs in the past year and who were HIV-positive were more likely to report combined physical and sexual abuse from intimate and commercial sexual partners [20]. Although these studies have identified specific HIV risk behaviors associated with histories of violence, few studies have focused on client-perpetrated violence specifically, and the causal links between violence and HIV-seropositivity have not been established.

Miller [21] developed a theoretical model in an effort to understand the relationship between history of sexual abuse and HIV risk among women. This model includes many of the sequelae associated with history of abuse including substance abuse, psychopathology (e.g., depression and PTSD), and sexual dissatisfaction and dysfunction (e.g., an inability to sustain intimate relationships, and participation in sex work). Miller proposed three possible causal pathways in the relation between sexual abuse and engaging in HIV-risky behavior: psychopathology as a result of abuse; drug use to cope with the abuse experience; and sexual adjustment problems related to taking sexual risks (e.g. obsession with sexual activity, participation in self-destructive sexual relationships, and sex work). Based upon Miller’s model, this study examines injection drug use as a mediator of the relationship between client-perpetrated violence and HIV-seropositivity among FSWs.

The specific links between history of abuse and HIV-seropositivity may not be entirely clear and may not be unidirectional, but some research exists on the relationship between violence victimization and increased needle sharing among IDUs. In a multi-site study of female IDUs in the US, Wagner and colleagues [22] found that IPV was associated with higher levels of syringe sharing. In a study of IDUs in Vancouver, Canada, 68% of women had a lifetime history of sexual violence, which was significantly associated with syringe sharing [23]. A previous study with IDUs in the same city showed that lifetime experience of sexual abuse was independently associated with syringe sharing among male and female IDUs [24]. However, these studies focused on lifetime experience of sexual violence, which could include either childhood or adulthood abuse, and results may not generalize to FSW populations currently experiencing abuse by clients. Therefore, further investigation of the relationship between recent client-perpetrated abuse, injection drug use, and HIV among FSWs is warranted.

This study utilized cross-sectional data to test injection drug use as a mediator of the relationship between client-perpetrated abuse and HIV-seropositivity among FSWs. We hypothesized that: (1) client-perpetrated abuse would be associated with HIV-seropositivity; (2) client-perpetrated abuse would be associated with injection drug use by FSWs; and (3) injection drug use would be associated with HIV-seropositivity. Mediation analysis allows for the examination of possible mechanisms through which independent variables affect dependent variables [25]. We conducted a mediation analysis to examine injection drug use as a possible mechanism by which client-perpetrated abuse may affect HIV-serostatus, specifically examining whether injection drug use mediates the relationship between client-perpetrated abuse and HIV-seropositivity. Since only 3% (n = 28) of FSW-IDUs in our sample reported sharing needles with their clients in the past 6 months, we chose to use injection drug use as the mediator instead of needle sharing.

Methods

Participants

Data were obtained from baseline interviews administered as part of an ongoing randomized trial of a safer sex intervention for FSWs in four Mexico-US border cities [26]. Baseline interview data from two of the four cities (Tijuana and Cd. Juarez) were available for use in this study. Participants were 924 FSWs from Tijuana and Cd. Juarez (n = 474 from Tijuana; n = 450 from Cd. Juarez). The eligibility criteria for this study were: age ≥ 18 years; traded sex for money, drugs, or other material benefits in the previous 2 months; and had unprotected sex with at least one client during the same time frame. Because one of the outcomes of the behavioral intervention was HIV incidence, FSWs who self-reported as HIV-positive at baseline were excluded from the study.

Procedures

Female sex workers (FSWs) residing in Tijuana and Cd. Juarez were recruited through street outreach, community and municipal health clinics, and referrals from participating FSWs. Interviews were conducted between January 2004 and March 2006. Women who completed the 60 min, interviewer-administered survey received $30 US dollars for their time. Study protocols were reviewed and approved by institutional review boards in San Diego, Tijuana, and Cd. Juarez.

Measures

The development of the questionnaire used in this study is described in detail elsewhere [27]. Client-perpetrated emotional, physical, and sexual abuse was assessed using three items selected from the family and social relationships section of the fifth edition of the Addiction Severity Index [28]. Participants responded either “yes” or “no” to whether they had experienced emotional, physical, and sexual abuse by a client in the past 6 months.

FSWs’ injection drug use was assessed by a single “yes/no” item asking participants if they injected drugs in the past 30 days.

HIV-status was ascertained using the Determine rapid HIV antibody test (Abbott Laboratories). Reactive samples were confirmed by EIA and Western blot. Pre- and post-test counseling was provided; participants with positive test results were referred to municipal health clinics for free medical care. Confirmatory HIV tests were conducted at either the San Diego County Health Department (for Tijuana samples) or the El Paso County Health Department (for Cd. Juarez samples).

Data Analyses

We compared FSWs who injected drugs in the past 30 days to those who did not, to identify any significant differences by IDU-status using Chi-square or Fisher’s exact test for categorical variables and t-tests for continuous variables. Next, we conducted mediation analysis according to the four steps suggested by Baron and Kenny [25] utilizing a series of logistic regression analyses to determine the relationship between: (1) the independent variables (each of the client abuse variables) and the dependent variable (HIV-seropositivity); (2) the independent variables and the proposed mediator (injection drug use); and (3) the proposed mediator and the dependent variable. To examine mediation, a final logistic regression was run with both the independent variable and the proposed mediator as predictors of the dependent variable. For a full mediating effect to exist, the impact of the independent variable on the dependent variable must be zero after the mediator is included in the model. A partial mediating effect exists when the impact of the independent variable on the dependent variable is significantly reduced when the proposed mediator is included in the model. Finally, a Sobel test [29] was conducted to determine the significance of the indirect effect of the mediator. The Sobel test results in a z-score consisting of the indirect beta coefficient divided by the indirect standard error which tests whether the indirect effect of the independent variable on the dependent variable via the mediator is significantly different from zero.

Results

Descriptive Statistics

The mean age of participants was 33.42 years (SD = 9.11); average length of time as a FSW was 6.44 years (SD = 6.74); and highest years of school completed was 6.14 (SD = 3.15). In our sample, 6% (n = 55) of the women were HIV-positive, and 12% (n = 114) reported injecting drugs in the past 30 days. The mean number of male clients in the past 6 months was 336 (SD = 325), and the median was 240 (min. = 1, max. = 1,920). Descriptive statistics for FSW-IDUs versus non-IDUs are presented in Table 1.

Table 1

Sociodemographic and client abuse characteristics by IDU vs. non-IDU status (N = 924)

	Non-IDU (n = 810)				FSW-IDU (n = 114)				Test statistic
	n	%	Mean	SD	n	%	Mean	SD	t	X 2
Age			33.77	9.34			30.98	6.83	3.07**
Years of school completed			6.07	3.15			6.65	3.09	−1.80
Years in sex work			6.30	6.93			6.88	5.82	−0.85
Number of male clients (past 6 months)			323.59	314.82			424.55	384.61	−3.11**
Has clients who have ever injected drugs	235	29.0			57	50.0				20.11***
Marital Status										23.58***
Married	20	2.5			3	2.6
Living with partner	157	19.4			42	36.8
Separated, Divorced, or Widowed	228	28.1			15	13.9
Single	401	49.5			53	46.5
Client-perpetrated emotional abuse (past 6 months)	194	24.0			43	37.7				9.83**
Client-perpetrated physical abuse (past 6 months)	133	16.4			30	26.3				6.67**
Client-perpetrated sexual abuse (past 6 months)	64	7.9			24	21.1				19.96***
HIV-Positive	40	4.9			14	12.3				9.79**

Note: Some frequencies do not sum to N due to missing values

* Significant at P < .05

** Significant at P < .01

*** Significant at P < .001

Prevalence of Abuse

In the total sample of FSWs, 26% reported experiencing emotional abuse by a client, 18% reported client-perpetrated physical abuse, and 10% reported client-perpetrated sexual abuse in the past 6 months. Among FSW-IDUs, 38% reported client-perpetrated emotional abuse, 26% reported client-perpetrated physical abuse, and 21% reported client-perpetrated sexual abuse in the past 6 months (see Table 1).

Bivariate Analyses

Characteristics of IDU versus non-IDU FSWs in this study are presented in greater detail elsewhere [30]. However, client-perpetrated abuse was not previously examined. Briefly, FSW-IDUs were younger (30.98 vs. 33.77 years), had more male clients in the past 6 months (424.55 vs. 323.59), and differed with respect to marital status: FSW-IDUs were more likely to be living with their romantic partner, while non-IDU FSWs were more likely to be single, separated, divorced, or widowed. FSW-IDUs were also more likely to have clients who had ever injected drugs (50 vs. 29%) and to have experienced client-perpetrated emotional abuse (38 vs. 24%), physical abuse (26 vs. 16%), or sexual abuse (21 vs. 8%) in the past 6 months, and to be HIV-positive (12 vs. 5%). The specific values and significance levels of the test statistics are presented in Table 1.

Mediation Analyses

Since FSW-IDUs had significantly more clients overall and more IDU clients than non-IDU FSWs, these variables were controlled for in the logistic regression analyses. Of the three types of abuse by clients, sexual abuse was significantly associated with HIV-seropositivity (b = 1.25, OR = 3.5, P < .001); whereas emotional and physical abuse were not. Hence, we conducted mediation analysis only for sexual abuse. As hypothesized, FSWs with higher rates of client-perpetrated sexual abuse were more likely to have injected drugs in the past 30 days (b = 1.09, OR = 3.0, P < .001), and injection drug use (controlling for the effect of sexual abuse) was positively associated with HIV-seropositivity (b = .82, OR = 2.26, P < .05).

FSWs’ injection drug use partially mediated the relationship between client-perpetrated sexual abuse and HIV-seropositivity. When both client-perpetrated sexual abuse and FSWs’ injection drug use were regressed simultaneously onto HIV-seropositivity, there was a decrease in the relationship between sexual abuse and seropositivity from b = 1.25, OR = 3.5, P < .001 to b = 1.10, OR = 3.0, P < .01 (see Fig. 1). An additional Sobel’s test of the indirect effect confirmed the significance of the mediator (z = 2.05, P < .05).

Fig. 1

FSWs injection drug use mediating the relationship between client-perpetrated sexual abuse and HIV-status. * P < .05, ** P < .01, *** P < .001

Intimate partner violence (IPV), childhood physical and sexual abuse, and unprotected vaginal and anal sex with clients were examined as potential confounds. FSW-IDUs did not differ from non-IDUs with respect to IPV, but they were more likely to have experienced childhood physical and sexual abuse. However, childhood physical and sexual abuse were not significantly associated with HIV status (r = .06 and .02, respectively); therefore, they were not controlled for in the regression analyses. FSW-IDUs did not differ from non-IDUs with respect to unprotected sex with clients, nor was this variable significantly associated with HIV status [30, 31]; therefore, it was deemed not necessary to control for this variable in the regression analyses, and injection drug use remained the variable of focus for mediation.

Discussion

Consistent with previous studies of FSWs, the women in our sample reported frequent experience of client-perpetrated abuse. Moreover, FSW-IDUs were significantly more likely to experience client-perpetrated emotional, physical, and sexual abuse and be HIV-positive as compared to their non-IDU counterparts. Being an IDU may be a critical marker in identifying FSWs who are at increased risk for violence victimization and infection with HIV. Our results showed that client-perpetrated abuse was significantly associated with a higher prevalence of injection drug use among FSWs, and those who experienced client-perpetrated sexual abuse were more likely to be HIV-positive, highlighting the need to consider history of abuse and injection drug use when assessing HIV risk among FSWs in this region.

As hypothesized, injection drug use partially mediated the relationship between client-perpetrated sexual abuse and HIV-status, suggesting that injection drug use may account for a significant proportion of this relationship. FSWs who are victims of violence may engage in drug use in order to numb themselves from the psychological consequences of the abuse and fear of revictimization. Wechsberg et al. [17] found that sex workers in South Africa with a history of abuse (childhood, IPV, and client-perpetrated) had a fear of future victimization and discomfort with the relationship dynamics with men, which in turn led to increased substance abuse and greater sexual risk. Additionally, FSWs may be more likely to acquiesce to requests for unsafe sex in order to earn more money to support their drug addiction [30].

Although this study identified injection drug use and history of client-perpetrated violence as important correlates of HIV risk among Mexican FSWs, it is likely that there is an underlying complexity of risk factors such as mental health disorders associated with trauma, gendered power dynamics, poverty, and societal norms and practices that contribute to FSWs’ overall risk [32-34]. Furthermore, these risks may be exacerbated by injection drug use. For example, a qualitative study among male and female IDUs in Tijuana and Cd. Juarez found that female IDUs often obtained and used drugs within the context of a sexual relationship with a drug-using, male romantic partner or spouse [35]. This could contribute to women’s receptive needle sharing or being “second on the needle,” [35, 36] which in turn may reflect a form of submission to their male partners [37]. These observations suggest that research with FSW-IDUs and their drug and sexual partners should take into consideration relationship dynamics and the intertwined nature of their drug use with sexual partnerships. Further research is warranted examining multi-level (i.e., micro-, meso-, and macro-level) factors of risk among this population. HIV prevention research targeting both FSWs and clients is urgently needed in order to adequately account for the clients’ sexual and drug risk taking and to identify potentially overlapping networks of high-risk drug- and sex-related partners who can be targeted for HIV prevention and harm reduction efforts [16, 38].

Although this study adds to the knowledge base on the relationship between client-perpetrated abuse, drug use, and HIV risk among FSWs, several limitations must be noted. First, because this study was cross-sectional, causal inferences cannot be drawn. Additional longitudinal studies that assess onset of abuse, injection drug use, and HIV-seropositivity may further clarify the observed associations. Second, the present study is retrospective and relies primarily on self-report data, which can entail recall bias. However, the recall period for the questions was relatively short (e.g., past 30 days to past 6 months), and previous research has provided validation for self-report data with IDU populations [39-41]. A third limitation was the limited, fixed nature of the abuse items selected from the Addiction Severity Index. These items did not assess multiple experiences of abuse, extent of abuse, or the number of different abusers, and therefore it may not reflect the true experience of client-perpetrated abuse for some of the FSWs in our study. Last, the results of this study may not be generalizable to other ethnic groups of FSWs, nor even to other Latina FSW groups. The participants in this study constituted convenience samples from two US-Mexico border cities and may not be representative of FSWs in other cities or of FSWs in either of those cities as a whole, especially since the intervention study deliberately recruited high-risk FSWs.

Further understanding of the relationship between client-perpetrated violence, injection drug use by FSWs, and FSWs’ HIV-risk behavior is important for the development of treatment and prevention strategies that adequately address the complexity of risks that FSW-IDUs face. Future HIV prevention interventions serving FSWs in this region should include violence screening and prevention as well as treatment for mental disorders, in order to address any negative sequelae of trauma and to help FSWs avoid potentially violent clients. The results of this study also suggest the need to treat victimized FSWs’ injection drug use, their impairment due to drug use, or both, in order to reduce the effect of client-perpetrated abuse on the risk of acquiring HIV. Increased access to drug treatment services may greatly enhance HIV prevention for this population. For example, Amaro and colleagues [42] found that treatment of drug addiction and trauma simultaneously was effective in reducing HIV sex-risk behaviors among poor inner-city Latina and African American women.

Finally, there is a need for HIV prevention interventions to move beyond exclusively individual-level approaches by including male clients in order to reduce women’s vulnerability to violence and HIV risks. A solely individual-level approach assumes that women can engage in preventive behaviors free from influence from their partners or larger societal norms [33]. However, research indicates that women’s HIV risk behavior is influenced by partner violence and power relationships that impact their ability to negotiate sexual and drug risk reduction behaviors [16–18, 22].