INTRODUCTION: Circulating plasma DNA is present in a considerably higher concentration in lung cancer patients than in controls. Conflicting data are reported about circulating DNA as a prognostic factor. The aim of this study was to prospectively analyse the relationship of circulating plasma DNA with overall survival (OS) of previously untreated non-small cell lung cancer (NSCLC) patients. METHODS: 46 untreated NSCLC patients and 21 controls with a follow-up time of 6.5 years were analyzed. Quantification of baseline circulating plasma DNA was performed by a real-time quantitative polymerase chain reaction (qPCR) targeting the human beta-globin gene. Survival analysis was performed using the Kaplan-Meier method and compared with a Cox-regression analysis. RESULTS: The median DNA concentration of the patients who died (87%) was significantly higher compared to the patients that survived at the end of follow-up (55ng/ml versus 23ng/ml, p=0.02). In patients with higher DNA concentration overall survival was significantly worse. In this study no relation of DNA concentration with tumour characteristics, age, gender or pulmonary inflammatory conditions was found. CONCLUSION: In this study a high circulating plasma DNA concentration at time of diagnosis in NSCLC patients was a prognostic factor for poorer survival. Circulating DNA may be used as a non-invasive biomarker to refine the prognostic profile in NSCLC patients. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
INTRODUCTION: Circulating plasma DNA is present in a considerably higher concentration in lung cancerpatients than in controls. Conflicting data are reported about circulating DNA as a prognostic factor. The aim of this study was to prospectively analyse the relationship of circulating plasma DNA with overall survival (OS) of previously untreated non-small cell lung cancer (NSCLC) patients. METHODS: 46 untreated NSCLCpatients and 21 controls with a follow-up time of 6.5 years were analyzed. Quantification of baseline circulating plasma DNA was performed by a real-time quantitative polymerase chain reaction (qPCR) targeting the human beta-globin gene. Survival analysis was performed using the Kaplan-Meier method and compared with a Cox-regression analysis. RESULTS: The median DNA concentration of the patients who died (87%) was significantly higher compared to the patients that survived at the end of follow-up (55ng/ml versus 23ng/ml, p=0.02). In patients with higher DNA concentration overall survival was significantly worse. In this study no relation of DNA concentration with tumour characteristics, age, gender or pulmonary inflammatory conditions was found. CONCLUSION: In this study a high circulating plasma DNA concentration at time of diagnosis in NSCLCpatients was a prognostic factor for poorer survival. Circulating DNA may be used as a non-invasive biomarker to refine the prognostic profile in NSCLCpatients. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
Authors: B T Li; A Drilon; M L Johnson; M Hsu; C S Sima; C McGinn; H Sugita; M G Kris; C G Azzoli Journal: Ann Oncol Date: 2015-10-20 Impact factor: 32.976
Authors: Avery Sonnenberg; Jennifer Y Marciniak; Elaine A Skowronski; Sareh Manouchehri; Laura Rassenti; Emanuela M Ghia; George F Widhopf; Thomas J Kipps; Michael J Heller Journal: Electrophoresis Date: 2014-05-14 Impact factor: 3.535
Authors: Avery Sonnenberg; Jennifer Y Marciniak; Laura Rassenti; Emanuela M Ghia; Elaine A Skowronski; Sareh Manouchehri; James McCanna; George F Widhopf; Thomas J Kipps; Michael J Heller Journal: Clin Chem Date: 2013-11-22 Impact factor: 8.327