BACKGROUND AND PURPOSE: We investigated the role of cyclo-oxygenase-2 (COX-2) in mechanisms of hyperbaric oxygen preconditioning (HBO-PC) in the mouse model of surgical brain injury (SBI). METHODS: C57BL mice were administered 100% oxygen for 1 hour at 2.5 atmosphere absolute for 5 consecutive days and subjected to SBI. Neurological status and brain edema were evaluated at 24 hours and 72 hours after the brain insult. Fluorescent immunostaining and Western blotting were performed to study hypoxia-inducible factor-1alpha and COX-2, respectively. Two doses of COX-2 inhibitor, NS398 (3 mg/kg and 10 mg/kg) were used to verify the role of COX-2 signaling pathway in the mechanism of HBO-PC. RESULTS: HBO-PC improved neurological status and decreased brain edema at 24 hours and 72 hours after SBI. HBO-PC by itself and SBI independently increased COX-2 levels by 2-fold and 4-fold, respectively. HBO-PC, however, reduced increase in hypoxia-inducible factor-1alpha and COX-2 expression after SBI. The HBO-PC-induced improvement in neurological status and brain edema was reversed by a suboptimal dose of the COX-2 inhibitor, NS398 (10 mg/kg intraperitoneally; 1/4th of dose shown to provide neuroprotection), which itself had no effect on investigated end points. CONCLUSIONS: HBO-PC attenuates postoperative brain edema and improves neurological outcomes after SBI. The HBO-PC-induced neuroprotection is mediated through COX-2 signaling pathways.
BACKGROUND AND PURPOSE: We investigated the role of cyclo-oxygenase-2 (COX-2) in mechanisms of hyperbaric oxygen preconditioning (HBO-PC) in the mouse model of surgical brain injury (SBI). METHODS: C57BL mice were administered 100% oxygen for 1 hour at 2.5 atmosphere absolute for 5 consecutive days and subjected to SBI. Neurological status and brain edema were evaluated at 24 hours and 72 hours after the brain insult. Fluorescent immunostaining and Western blotting were performed to study hypoxia-inducible factor-1alpha and COX-2, respectively. Two doses of COX-2 inhibitor, NS398 (3 mg/kg and 10 mg/kg) were used to verify the role of COX-2 signaling pathway in the mechanism of HBO-PC. RESULTS:HBO-PC improved neurological status and decreased brain edema at 24 hours and 72 hours after SBI. HBO-PC by itself and SBI independently increased COX-2 levels by 2-fold and 4-fold, respectively. HBO-PC, however, reduced increase in hypoxia-inducible factor-1alpha and COX-2 expression after SBI. The HBO-PC-induced improvement in neurological status and brain edema was reversed by a suboptimal dose of the COX-2 inhibitor, NS398 (10 mg/kg intraperitoneally; 1/4th of dose shown to provide neuroprotection), which itself had no effect on investigated end points. CONCLUSIONS:HBO-PC attenuates postoperative brain edema and improves neurological outcomes after SBI. The HBO-PC-induced neuroprotection is mediated through COX-2 signaling pathways.
Authors: Steve Lee; Vikram Jadhav; Robert E Ayer; Hugo Rojas; Amy Hyong; Tim Lekic; Jiping Tang; John H Zhang Journal: J Pineal Res Date: 2008-06-18 Impact factor: 13.007
Authors: Robert P Ostrowski; Gerhart Graupner; Elena Titova; Jennifer Zhang; Jeffrey Chiu; Neal Dach; Dalia Corleone; Jiping Tang; John H Zhang Journal: Neurobiol Dis Date: 2007-07-28 Impact factor: 5.996
Authors: Zhiyong Qin; Shuijiang Song; Guohua Xi; Robert Silbergleit; Richard F Keep; Julian T Hoff; Ya Hua Journal: Neurosurg Focus Date: 2007-05-15 Impact factor: 4.047
Authors: Cherine H Kim; Devin W McBride; Prativa Sherchan; Carl E Person; Eric C K Gren; Wayne Kelln; Tim Lekic; William K Hayes; Jiping Tang; John H Zhang Journal: Neurobiol Dis Date: 2017-03-09 Impact factor: 5.996
Authors: Shoji Yokobori; Anna T Mazzeo; Khadil Hosein; Shyam Gajavelli; W Dalton Dietrich; M Ross Bullock Journal: Transl Stroke Res Date: 2012-11-15 Impact factor: 6.829