| Literature DB >> 19616504 |
Akiko Yamada1, Takenori Yamamoto, Yuya Yoshimura, Shunichi Gouda, Satoshi Kawashima, Naoshi Yamazaki, Kikuji Yamashita, Masatoshi Kataoka, Toshihiko Nagata, Hiroshi Terada, Douglas R Pfeiffer, Yasuo Shinohara.
Abstract
Yeast mitochondria have generally been believed not to undergo the permeability transition (PT) by the accumulation of Ca(2+) within the mitochondrial matrix, unlike mammalian mitochondria. However, the reason why the yeast PT is not induced by Ca(2+) has remained obscure. In this study, we examined in detail the effects of Ca(2+) on yeast mitochondria under various conditions. As a result, we discovered that the PT could be induced even in yeast mitochondria by externally added Ca(2+) under optimized experimental conditions. The 2 essential parameters for proper observation of the PT-inducing effects of Ca(2+) were the concentrations of the respiratory substrate and that of inorganic phosphate (Pi) in the incubation medium. The yeast mitochondrial PT induced by Ca(2+) was found to be insensitive to cyclosporin A and suppressed in the presence of a high concentration of Pi. Furthermore, when the PT was induced in yeast mitochondria by Ca(2+), the release of cytochrome c from mitochondria was also observed.Entities:
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Year: 2009 PMID： 19616504 DOI： 10.1016/j.bbabio.2009.07.001
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002