PURPOSE: To compare closed-system solid surface vitrification with slow freezing. METHODS: Mouse 2-cell embryos (n = 348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% fetal bovine serum (FBS) in phosphate-buffered saline (PBS) for 10 min, then transferred into 17.5% EG, 17.5% DMSO, 0.25 M trehalose and 10% FBS in PBS. They were placed on hemi-straws and inserted into 0.5 ml straws inside a previously cooled aluminum cylinder. Slow freezing was done in straws by the conventional method. RESULTS: Vitrified embryos had significantly higher survival, further cleavage and blastocyst formation rates than those in the slow freezing group (p < 0.001) and were comparable to controls. Blastocysts in the vitrification and control groups had significantly more cells than those in the slow freezing group (p < 0.05). CONCLUSIONS: Closed-system vitrification was more effective than conventional slow freezing.
PURPOSE: To compare closed-system solid surface vitrification with slow freezing. METHODS:Mouse 2-cell embryos (n = 348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% fetal bovine serum (FBS) in phosphate-buffered saline (PBS) for 10 min, then transferred into 17.5% EG, 17.5% DMSO, 0.25 M trehalose and 10% FBS in PBS. They were placed on hemi-straws and inserted into 0.5 ml straws inside a previously cooled aluminum cylinder. Slow freezing was done in straws by the conventional method. RESULTS: Vitrified embryos had significantly higher survival, further cleavage and blastocyst formation rates than those in the slow freezing group (p < 0.001) and were comparable to controls. Blastocysts in the vitrification and control groups had significantly more cells than those in the slow freezing group (p < 0.05). CONCLUSIONS: Closed-system vitrification was more effective than conventional slow freezing.
Authors: Juergen Liebermann; Michael J Tucker; James R Graham; Taylor Han; Alana Davis; Michael J Levy Journal: Reprod Biomed Online Date: 2002 Mar-Apr Impact factor: 3.828
Authors: Bence Somoskoi; Nicola A Martino; Rosa A Cardone; Giovanni M Lacalandra; Maria E Dell'Aquila; Sandor Cseh Journal: Reprod Biol Endocrinol Date: 2015-03-24 Impact factor: 5.211
Authors: Nicola A Martino; Maria E Dell'aquila; Rosa A Cardone; Bence Somoskoi; Giovanni M Lacalandra; Sandor Cseh Journal: Reprod Biol Endocrinol Date: 2013-04-03 Impact factor: 5.211