BACKGROUND: Low-grade inflammation may potentially explain the relationship between obstructive sleep apnea syndrome (OSA) and cardiovascular events. However, the respective contribution of intermittent hypoxia and confounders, such as obesity, is still debated. OBJECTIVES: To monitor urinary leukotriene E(4) (U-LTE(4)), a validated marker of proinflammatory cysteinyl leukotriene production, in OSA; to determine the influence of obesity and other confounders on U-LTE(4) concentrations; to examine the mechanisms involved through transcriptional profiling of the leukotriene pathway in peripheral blood mononuclear cells (PBMCs); and to investigate the effect of continuous positive air pressure (CPAP) on U-LTE(4) concentrations. METHODS: We measured U-LTE(4) by liquid chromatography-tandem mass spectrometry. RESULTS: The U-LTE(4) concentrations were increased (P = .019) in 40 nonobese patients with OSA carefully matched for age, sex, and body mass index (BMI) to 25 control subjects, and correlated (r = 0.0312; P = .017) to the percentage of time spent with mean oxygen saturation (SaO(2)) less than 90%. In a larger cohort of patients with OSA (n = 72), U-LTE(4) increased as a function of BMI (r = 0.445; P = .0002). In those patients, the expression levels of 5-lipoxygenase activating protein mRNA in mononuclear cells exhibited a similar pattern. A stepwise multiple linear regression analysis performed in this cohort identified BMI (P = .001; regression coefficient, 3.33) and percentage of time spent with SaO(2) <90% (P = .001; regression coefficient, 1.01) as independent predictors of U-LTE(4) concentrations. Compared with baseline, CPAP reduced by 22% (P = .006) U-LTE(4) concentrations only in patients with OSA with normal BMI. CONCLUSION: Obesity, and to a lesser extent hypoxia severity, are determinant of U-LTE(4) production in patients with OSA.
BACKGROUND: Low-grade inflammation may potentially explain the relationship between obstructive sleep apnea syndrome (OSA) and cardiovascular events. However, the respective contribution of intermittent hypoxia and confounders, such as obesity, is still debated. OBJECTIVES: To monitor urinary leukotriene E(4) (U-LTE(4)), a validated marker of proinflammatory cysteinyl leukotriene production, in OSA; to determine the influence of obesity and other confounders on U-LTE(4) concentrations; to examine the mechanisms involved through transcriptional profiling of the leukotriene pathway in peripheral blood mononuclear cells (PBMCs); and to investigate the effect of continuous positive air pressure (CPAP) on U-LTE(4) concentrations. METHODS: We measured U-LTE(4) by liquid chromatography-tandem mass spectrometry. RESULTS: The U-LTE(4) concentrations were increased (P = .019) in 40 nonobese patients with OSA carefully matched for age, sex, and body mass index (BMI) to 25 control subjects, and correlated (r = 0.0312; P = .017) to the percentage of time spent with mean oxygen saturation (SaO(2)) less than 90%. In a larger cohort of patients with OSA (n = 72), U-LTE(4) increased as a function of BMI (r = 0.445; P = .0002). In those patients, the expression levels of 5-lipoxygenase activating protein mRNA in mononuclear cells exhibited a similar pattern. A stepwise multiple linear regression analysis performed in this cohort identified BMI (P = .001; regression coefficient, 3.33) and percentage of time spent with SaO(2) <90% (P = .001; regression coefficient, 1.01) as independent predictors of U-LTE(4) concentrations. Compared with baseline, CPAP reduced by 22% (P = .006) U-LTE(4) concentrations only in patients with OSA with normal BMI. CONCLUSION:Obesity, and to a lesser extent hypoxia severity, are determinant of U-LTE(4) production in patients with OSA.
Authors: Françoise Stanke-Labesque; Jean-Louis Pépin; Tiphaine de Jouvencel; Claire Arnaud; Jean-Philippe Baguet; Marcelo H Petri; Renaud Tamisier; Jean François Jourdil; Patrick Lévy; Magnus Bäck Journal: J Lipid Res Date: 2012-07-03 Impact factor: 5.922
Authors: Magnus Bäck; William S Powell; Sven-Erik Dahlén; Jeffrey M Drazen; Jilly F Evans; Charles N Serhan; Takao Shimizu; Takehiko Yokomizo; G Enrico Rovati Journal: Br J Pharmacol Date: 2014-07-12 Impact factor: 8.739
Authors: Magnus Bäck; Antoine Avignon; Françoise Stanke-Labesque; Catherine Boegner; Vincent Attalin; Elodie Leprieur; Ariane Sultan Journal: PLoS One Date: 2014-12-01 Impact factor: 3.240
Authors: Elena Nobili; M Dolores Salvado; Lasse Folkersen; Laura Castiglioni; Jens Kastrup; Anders Wetterholm; Elena Tremoli; Göran K Hansson; Luigi Sironi; Jesper Z Haeggström; Anders Gabrielsen Journal: PLoS One Date: 2012-07-25 Impact factor: 3.240