BACKGROUND/AIMS: We investigated, for the first time, the protective effect of erythropoietin (EPO) against liver ischemia-reperfusion (I-R) injury in a pig model. METHODOLOGY: Partial hepatic ischemia was maintained for 60 min in a pig. Pigs were allocated to 4 groups (n=5 each): (1) Control group with I-R injury (Vehicle); (2) EPO group with I-R injury, given three injections of EPO at 5000 IU/kg (EPO5000x3); (3) EPO group with I-R injury, given a single injection of EPO at 5000 IU/kg (EPO5000x1); and (4) EPO group with I-R injury, given three injections of EPO at 500 IU/kg (EPO500x3). Liver function tests (AST, ALT, LDH), and TUNEL assay were performed. RESULTS: Three hours after I-R injury, AST levels in the Vehicle, EPO5000x3, EPO5000x1, and EPO500 x3 groups were 1494.2 +/- 711.3 U/L, 307.3 +/- 127.6 UL, 296.5 +/- 9.2 U/L, and 474.6 +/- 242.0 UL, respectively (one-factor ANOVA, p = 0.020). At 3h the ALT and LDH levels in the Vehicle group were significantly higher than those in the EPO5000x3 and EPO5000x1 groups. Apoptotic indices in the Vehicle, EPO500x3, EPO5000x1, and EPO500x3 groups 3 h after I-R injury were 2.40 +/- 0.93, 1.36 +/- 0.12, 1.11 +/- 0.17, and 1.51 +/- 0.33, respectively. The apoptotic indices of the EPO5000x1 and EPO500x3 groups were significantly lower than that of the Vehicle group. CONCLUSIONS: EPO treatment significantly ameliorated liver I-R injury in this pig model. The protective effect was exerted by the inhibition of apoptosis. These results will open the door for the clinical application of EPO in liver surgery.
BACKGROUND/AIMS: We investigated, for the first time, the protective effect of erythropoietin (EPO) against liver ischemia-reperfusion (I-R) injury in a pig model. METHODOLOGY: Partial hepatic ischemia was maintained for 60 min in a pig. Pigs were allocated to 4 groups (n=5 each): (1) Control group with I-R injury (Vehicle); (2) EPO group with I-R injury, given three injections of EPO at 5000 IU/kg (EPO5000x3); (3) EPO group with I-R injury, given a single injection of EPO at 5000 IU/kg (EPO5000x1); and (4) EPO group with I-R injury, given three injections of EPO at 500 IU/kg (EPO500x3). Liver function tests (AST, ALT, LDH), and TUNEL assay were performed. RESULTS: Three hours after I-R injury, AST levels in the Vehicle, EPO5000x3, EPO5000x1, and EPO500 x3 groups were 1494.2 +/- 711.3 U/L, 307.3 +/- 127.6 UL, 296.5 +/- 9.2 U/L, and 474.6 +/- 242.0 UL, respectively (one-factor ANOVA, p = 0.020). At 3h the ALT and LDH levels in the Vehicle group were significantly higher than those in the EPO5000x3 and EPO5000x1 groups. Apoptotic indices in the Vehicle, EPO500x3, EPO5000x1, and EPO500x3 groups 3 h after I-R injury were 2.40 +/- 0.93, 1.36 +/- 0.12, 1.11 +/- 0.17, and 1.51 +/- 0.33, respectively. The apoptotic indices of the EPO5000x1 and EPO500x3 groups were significantly lower than that of the Vehicle group. CONCLUSIONS:EPO treatment significantly ameliorated liver I-R injury in this pig model. The protective effect was exerted by the inhibition of apoptosis. These results will open the door for the clinical application of EPO in liver surgery.
Authors: Nadia Karina Guimarães-Souza; Liliya Marsovna Yamaleyeva; Baisong Lu; Ana Claudia Mallet de Souza Ramos; Colin Edward Bishop; Karl Erik Andersson Journal: Einstein (Sao Paulo) Date: 2015 Jan-Mar
Authors: Maria Chalasti; Christos Iordanou; Zisis Kratiras; Aikaterini Stylianaki; Eleni-Andriana Trigka; Eleftheria Lakiotaki; Kali Makedou; Stavros Iliadis; Konstantinos G Zografos; Dimitrios Dimitroulis; Michail Chrisofos; Efstratios Patsouris; Georgios C Zografos; George C Bouboulis; Apostolos E Papalois Journal: J Int Med Res Date: 2020-06 Impact factor: 1.671