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Literature DB >> 19578383

Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions.

Nelson P Barrera¹, Shoshanna C Isaacson, Min Zhou, Vassiliy N Bavro, Alex Welch, Theresia A Schaedler, Markus A Seeger, Ricardo Núñez Miguel, Vladimir M Korkhov, Hendrik W van Veen, Henrietta Venter, Adrian R Walmsley, Christopher G Tate, Carol V Robinson.

Abstract

We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex to multiple collisions, we released the intact complex largely devoid of detergent. This enabled us to characterize both subunit stoichiometry and lipid binding in 4 membrane protein complexes.

Entities: Chemical

Mesh：

Substances：

Year: 2009 PMID： 19578383 PMCID： PMC4066579 DOI： 10.1038/nmeth.1347

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

24 in total

Review 1. Comparison of three structures of the multidrug transporter EmrE.

Authors: Christopher G Tate
Journal: Curr Opin Struct Biol Date: 2006-07-07 Impact factor: 6.809

2. On the energy-dependence of Hoechst 33342 transport by the ABC transporter LmrA.

Authors: Henrietta Venter; Saroj Velamakanni; Lekshmy Balakrishnan; Hendrik W van Veen
Journal: Biochem Pharmacol Date: 2007-10-26 Impact factor: 5.858

Review 3. Molecular basis of multidrug transport by ABC transporters.

Authors: Markus A Seeger; Hendrik W van Veen
Journal: Biochim Biophys Acta Date: 2008-12-24

4. The purified and functionally reconstituted multidrug transporter LmrA of Lactococcus lactis mediates the transbilayer movement of specific fluorescent phospholipids.

Authors: A Margolles; M Putman; H W van Veen; W N Konings
Journal: Biochemistry Date: 1999-12-07 Impact factor: 3.162

5. Expression of genes associated with antibiotic extrusion in Pseudomonas aeruginosa.

Authors: S R Morshed; Y Lei; H Yoneyama; T Nakae
Journal: Biochem Biophys Res Commun Date: 1995-05-16 Impact factor: 3.575

6. JOY: protein sequence-structure representation and analysis.

Authors: K Mizuguchi; C M Deane; T L Blundell; M S Johnson; J P Overington
Journal: Bioinformatics Date: 1998 Impact factor: 6.937

7. EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents.

Authors: H Yerushalmi; M Lebendiker; S Schuldiner
Journal: J Biol Chem Date: 1995-03-24 Impact factor: 5.157

8. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin.

Authors: P G de Ruyter; O P Kuipers; W M de Vos
Journal: Appl Environ Microbiol Date: 1996-10 Impact factor: 4.792

9. Micelles protect membrane complexes from solution to vacuum.

Authors: Nelson P Barrera; Natalie Di Bartolo; Paula J Booth; Carol V Robinson
Journal: Science Date: 2008-06-12 Impact factor: 47.728

10. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.

Authors: H Schägger; G von Jagow
Journal: Anal Biochem Date: 1991-12 Impact factor: 3.365

76 in total

1. Insights into the Basal Activity and Activation Mechanism of the β1 Adrenergic Receptor Using Native Mass Spectrometry.

Authors: Agni F M Gavriilidou; Hanna Hunziker; Daniel Mayer; Ziva Vuckovic; Dmitry B Veprintsev; Renato Zenobi
Journal: J Am Soc Mass Spectrom Date: 2018-12-03 Impact factor: 3.109

9. Traveling-wave Ion Mobility-Mass Spectrometry Reveals Additional Mechanistic Details in the Stabilization of Protein Complex Ions through Tuned Salt Additives.

Authors: Linjie Han; Brandon T Ruotolo
Journal: Int J Ion Mobil Spectrom Date: 2013-01-29

10. Native mass spectrometry characterization of intact nanodisc lipoprotein complexes.

Authors: Michael T Marty; Hao Zhang; Weidong Cui; Robert E Blankenship; Michael L Gross; Stephen G Sligar
Journal: Anal Chem Date: 2012-10-17 Impact factor: 6.986

Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions.

Review 1. Comparison of three structures of the multidrug transporter EmrE.

2. On the energy-dependence of Hoechst 33342 transport by the ABC transporter LmrA.

Review 3. Molecular basis of multidrug transport by ABC transporters.

4. The purified and functionally reconstituted multidrug transporter LmrA of Lactococcus lactis mediates the transbilayer movement of specific fluorescent phospholipids.

5. Expression of genes associated with antibiotic extrusion in Pseudomonas aeruginosa.

6. JOY: protein sequence-structure representation and analysis.

7. EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents.

8. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin.

9. Micelles protect membrane complexes from solution to vacuum.

10. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.

1. Insights into the Basal Activity and Activation Mechanism of the β1 Adrenergic Receptor Using Native Mass Spectrometry.

2. Reliable determinations of protein-ligand interactions by direct ESI-MS measurements. Are we there yet?

3. Norwalk virus assembly and stability monitored by mass spectrometry.

4. How far can we go with structural mass spectrometry of protein complexes?

5. Current limitations in native mass spectrometry based structural biology.

6. Do charge state signatures guarantee protein conformations?

7. Ion mobility-mass spectrometry of a rotary ATPase reveals ATP-induced reduction in conformational flexibility.

Review 8. Top Down proteomics: facts and perspectives.

9. Traveling-wave Ion Mobility-Mass Spectrometry Reveals Additional Mechanistic Details in the Stabilization of Protein Complex Ions through Tuned Salt Additives.

10. Native mass spectrometry characterization of intact nanodisc lipoprotein complexes.