Literature DB >> 19574654

Purification, crystallization and preliminary crystallographic studies of a Kunitz-type proteinase inhibitor from tamarind (Tamarindus indica) seeds.

Dipak N Patil1, Anshul Chaudhry, Ashwani K Sharma, Shailly Tomar, Pravindra Kumar.   

Abstract

A Kunitz-type proteinase inhibitor has been purified from tamarind (Tamarindus indica) seeds. SDS-PAGE analysis of a purified sample showed a homogeneous band corresponding to a molecular weight of 21 kDa. The protein was identified as a Kunitz-type proteinase inhibitor based on N-terminal amino-acid sequence analysis. It was crystallized by the vapour-diffusion method using PEG 6000. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 37.2, b = 77.1, c = 129.1 A. Diffraction data were collected to a resolution of 2.7 A. Preliminary crystallographic analysis indicated the presence of one proteinase inhibitor molecule in the asymmetric unit, with a solvent content of 44%.

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Year:  2009        PMID： 19574654      PMCID： PMC2705649          DOI： 10.1107/S1744309109023495

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

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Journal:  J Agric Food Chem       Date:  2005-06-01       Impact factor: 5.279

9.  Abundant class III acidic chitinase homologue in tamarind (Tamarindus indica) seed serves as the major storage protein.

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Authors:  Chandini S Kumar; Sila Bhattacharya
Journal:  Crit Rev Food Sci Nutr       Date:  2008-01       Impact factor: 11.176
  10 in total
  4 in total

1.  Crystallization and preliminary X-ray diffraction analysis of the complex of Kunitz-type tamarind trypsin inhibitor and porcine pancreatic trypsin.

Authors:  Sakshi Tomar; Dipak N Patil; Manali Datta; Satya Tapas; Anshul Chaudhary; Ashwani K Sharma; Shailly Tomar; Pravindra Kumar
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-10-30

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Journal:  PLoS One       Date:  2016-11-15       Impact factor: 3.240

3.  Trypsin inhibitors demonstrate antioxidant activities, inhibit A549 cell proliferation, and increase activities of reactive oxygen species scavenging enzymes.

Authors:  Tooba Naz Shamsi; Romana Parveen; Sadaf Fatima
Journal:  Indian J Pharmacol       Date:  2017 Mar-Apr       Impact factor: 1.200

4.  A novel function for globulin in sequestering plant hormone: Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin.

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Journal:  Sci Rep       Date:  2017-07-05       Impact factor: 4.379
  4 in total

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