Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas.

Literature DB >> 19574652

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas.

Shabir Najmudin¹, Cecília Bonifácio, Américo G Duarte, Sofia R Pauleta, Sofia R Pualeta, Isabel Moura, José J G Moura, Maria J Romão.

Abstract

The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 A.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 2009 PMID： 19574652 PMCID： PMC2705647 DOI： 10.1107/S1744309109023392

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

14 in total

1. SOLVE and RESOLVE: automated structure solution, density modification and model building.

Authors: Thomas Terwilliger
Journal: J Synchrotron Radiat Date: 2003-11-28 Impact factor: 2.616

2. NMR structure determination and structure-based functional characterization of conserved hypothetical protein MTH1175 from Methanobacterium thermoautotrophicum.

Authors: J R Cort; A Yee; A M Edwards; C H Arrowsmith; M A Kennedy
Journal: J Struct Funct Genomics Date: 2000

3. Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas.

Authors: S A Bursakov; O Yu Gavel; G Di Rocco; J Lampreia; J Calvete; A S Pereira; J J G Moura; I Moura
Journal: J Inorg Biochem Date: 2004-05 Impact factor: 4.155

4. NMR structure determination of the hypothetical protein TM1290 from Thermotoga maritima using automated NOESY analysis.

Authors: Touraj Etezady-Esfarjani; Torsten Herrmann; Wolfgang Peti; Heath E Klock; Scott A Lesley; Kurt Wüthrich
Journal: J Biomol NMR Date: 2004-07 Impact factor: 2.835

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas.

1. SOLVE and RESOLVE: automated structure solution, density modification and model building.

2. NMR structure determination and structure-based functional characterization of conserved hypothetical protein MTH1175 from Methanobacterium thermoautotrophicum.

3. Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas.

4. NMR structure determination of the hypothetical protein TM1290 from Thermotoga maritima using automated NOESY analysis.

5. Towards complete validated models in the next generation of ARP/wARP.

6. NMR structure determination of the conserved hypothetical protein TM1816 from Thermotoga maritima.

7. NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster.

8. Solvent content of protein crystals.

Review 9. Scaling and assessment of data quality.

10. BALBES: a molecular-replacement pipeline.

1. Orange protein from Desulfovibrio alaskensis G20: insights into the Mo-Cu cluster protein-assisted synthesis.