R Nandakumar1, N Madayiputhiya, A F Fouad. 1. Department of Endodontics, Prosthodontics and Operative Dentistry, Baltimore College of Dental Surgery, Baltimore, MD, USA.
Abstract
INTRODUCTION: Endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. Proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. The purpose of this study was to apply proteomic techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the identification of proteins of bacterial origin present in endodontic infections. METHODS: Endodontic specimens were aseptically obtained from seven patients with root canal infections. Protein mixtures were subjected to tryptic in-solution digestion and analysed by reverse-phase nano-LC-MS/MS followed by a database search. RESULTS: Proteins, mainly of cell wall or membrane origin, from endodontic bacteria especially Enterococcus faecalis, Enterococcus faecium, Porphyromonas gingivalis, Fusobacterium nucleatum, and Treponema denticola were identified from all the samples tested. Identified proteins included adhesins, autolysins, proteases, virulence factors, and antibiotic-resistance proteins. CONCLUSIONS: LC-MS/MS offers a sensitive analytical platform to study the disease processes in the root canal environment. The array of proteins expressed in endodontic infections reflects the complex microbial presence and highlights the bacterial species involved in the inflammatory process.
INTRODUCTION:Endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. Proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. The purpose of this study was to apply proteomic techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the identification of proteins of bacterial origin present in endodontic infections. METHODS: Endodontic specimens were aseptically obtained from seven patients with root canal infections. Protein mixtures were subjected to tryptic in-solution digestion and analysed by reverse-phase nano-LC-MS/MS followed by a database search. RESULTS: Proteins, mainly of cell wall or membrane origin, from endodontic bacteria especially Enterococcus faecalis, Enterococcus faecium, Porphyromonas gingivalis, Fusobacterium nucleatum, and Treponema denticola were identified from all the samples tested. Identified proteins included adhesins, autolysins, proteases, virulence factors, and antibiotic-resistance proteins. CONCLUSIONS: LC-MS/MS offers a sensitive analytical platform to study the disease processes in the root canal environment. The array of proteins expressed in endodontic infections reflects the complex microbial presence and highlights the bacterial species involved in the inflammatory process.
Authors: Ruby Singh; O Colin Stine; David L Smith; John K Spitznagel; Mohamed E Labib; Henry N Williams Journal: Appl Environ Microbiol Date: 2003-06 Impact factor: 4.792
Authors: Purnima S Kumar; Eugene J Leys; Jennifer M Bryk; Francisco J Martinez; Melvin L Moeschberger; Ann L Griffen Journal: J Clin Microbiol Date: 2006-10 Impact factor: 5.948
Authors: Ying Ge; Mariam El-Naggar; Siu Kwan Sze; Han Bin Oh; Tadhg P Begley; Fred W McLafferty; Helena Boshoff; Clifton E Barry Journal: J Am Soc Mass Spectrom Date: 2003-03 Impact factor: 3.109
Authors: Yulanda M Williamson; Hercules Moura; David Schieltz; Jon Rees; Adrian R Woolfitt; James L Pirkle; Jacquelyn S Sampson; Maria L Tondella; Edwin Ades; George Carlone; John R Barr Journal: J Biomed Biotechnol Date: 2010-05-23
Authors: José Chibebe Junior; Beth B Fuchs; Caetano P Sabino; Juliana C Junqueira; Antonio O C Jorge; Martha S Ribeiro; Michael S Gilmore; Louis B Rice; George P Tegos; Michael R Hamblin; Eleftherios Mylonakis Journal: PLoS One Date: 2013-02-14 Impact factor: 3.240
Authors: José Claudio Provenzano; José F Siqueira; Isabela N Rôças; Romênia R Domingues; Adriana F Paes Leme; Márcia R S Silva Journal: PLoS One Date: 2013-10-15 Impact factor: 3.240