Literature DB >> 19563122

Measuring DNA content by flow cytometry in fission yeast.

Sarah A Sabatinos1, Susan L Forsburg.   

Abstract

Flow cytometry is an essential tool to monitor DNA content and determine cell cycle distribution. Its utility reflects the relative ease of sample preparation and the stochiometric nature of the most popular DNA-binding dyes (propidium iodide and Sytox Green). Mammalian precedents using flow cytometry for replication and cell biology studies are attractive examples for S. pombe researchers. However, the study of DNA replication with multicolor analysis has lagged behind that in mammalian cells. We present basic and advanced protocols for analysis of DNA replication in fission yeast by flow cytometry including whole cell, nuclear "ghosts," and two-color imaging with BrdU.

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Year:  2009        PMID： 19563122     DOI： 10.1007/978-1-60327-815-7_25

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  28 in total

1.  The Rho1 GTPase-activating protein CgBem2 is required for survival of azole stress in Candida glabrata.

Authors:  Sapan Borah; Raju Shivarathri; Rupinder Kaur
Journal:  J Biol Chem       Date:  2011-08-08       Impact factor: 5.157

2.  An antisense RNA-mediated mechanism eliminates a meiosis-specific copper-regulated transcript in mitotic cells.

Authors:  Vincent Normant; Jude Beaudoin; Simon Labbé
Journal:  J Biol Chem       Date:  2015-07-30       Impact factor: 5.157

3.  Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.

Authors:  Lubos Cipak; Silvia Polakova; Randy W Hyppa; Gerald R Smith; Juraj Gregan
Journal:  Nat Protoc       Date:  2014-01-02       Impact factor: 13.491

4.  Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.

Authors:  Lin Ding; Susan L Forsburg
Journal:  J Biol Chem       Date:  2011-08-03       Impact factor: 5.157

5.  Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination.

Authors:  Samuel Plante; Vincent Normant; Karla M Ramos-Torres; Simon Labbé
Journal:  J Biol Chem       Date:  2017-06-01       Impact factor: 5.157

6.  Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).

Authors:  Tara L Mastro; Susan L Forsburg
Journal:  Genetics       Date:  2014-10-06       Impact factor: 4.562

7.  Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe.

Authors:  Lubos Cipak; Chao Zhang; Ines Kovacikova; Cornelia Rumpf; Eva Miadokova; Kevan M Shokat; Juraj Gregan
Journal:  Cell Cycle       Date:  2011-10-15       Impact factor: 4.534

8.  Fission yeast TOR signaling is essential for the down-regulation of a hyperactivated stress-response MAP kinase under salt stress.

Authors:  Junpei Ishiguro; Kenta Shibahara; Yumi Ueda; Kei Nakamura
Journal:  Mol Genet Genomics       Date:  2012-12-28       Impact factor: 3.291

9.  Spore Germination Requires Ferrichrome Biosynthesis and the Siderophore Transporter Str1 in Schizosaccharomyces pombe.

Authors:  Samuel Plante; Simon Labbé
Journal:  Genetics       Date:  2019-01-15       Impact factor: 4.562

10.  A mammalian-like DNA damage response of fission yeast to nucleoside analogs.

Authors:  Sarah A Sabatinos; Tara L Mastro; Marc D Green; Susan L Forsburg
Journal:  Genetics       Date:  2012-11-12       Impact factor: 4.562
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