Literature DB >> 19562498

Plastid transformation in eggplant (Solanum melongena L.).

A K Singh1, S S Verma, K C Bansal.   

Abstract

We have developed a method for plastid transformation in eggplant (Solanum melongena L.), a solanaceous plant species. Plastid transformation in eggplant was achieved by bombardment of green stem segments with pPRV111A plastid expression vector carrying the aadA gene encoding aminoglycoside 3''-adenylyltransferase. Biolistic delivery of the pPRV111A plasmid yielded transplastomic plants at a frequency of two per 21 bombarded plates containing 25 stem explants each. Integration of the aadA gene in the plastome was verified by PCR analysis and also by Southern blotting using 16S rDNA (targeting sequence) and the aadA gene as a probe. Transplastomic expression of the aadA gene was verified by RT-PCR. The development of transplastomic technology in eggplant may open up exciting possibilities for novel gene introduction and expression in the engineered plastome for agronomic or pharmaceutical traits.

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Year:  2009        PMID： 19562498     DOI： 10.1007/s11248-009-9290-z

Source DB:  PubMed          Journal:  Transgenic Res        ISSN： 0962-8819            Impact factor:   2.788


  27 in total

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Journal:  Plant Cell Rep       Date:  1990-06       Impact factor: 4.570

10.  Agrobacterium mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. 'Kecskeméti lila').

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  15 in total

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4.  Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.

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Journal:  Protoplasma       Date:  2012-03-07       Impact factor: 3.356

6.  Plant plastid engineering.

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Review 8.  Chloroplast genomes: diversity, evolution, and applications in genetic engineering.

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9.  Stable plastid transformation for high-level recombinant protein expression: promises and challenges.

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Review 10.  Plastid genomics in horticultural species: importance and applications for plant population genetics, evolution, and biotechnology.

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