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Literature DB >> 15604694

Generation of fertile transplastomic soybean.

Nathalie Dufourmantel¹, Bernard Pelissier, Frederic Garçon, Gilles Peltier, Jean-Marc Ferullo, Ghislaine Tissot.

Abstract

We describe here the development of a plastid transformation method for soybean, a leguminous plant of major agronomic interest. Chloroplasts from embryogenic tissue of Glycine max have been successfully transformed by bombardment. The transforming DNA carries a spectinomycin resistance gene (aadA) under the control of tobacco plastid regulatory expression elements, flanked by two adjacent soybean plastome sequences allowing its targeted insertion between the trnV gene and the rps12/7 operon. All generated spectinomycin resistant plants were transplastomic and no remaining wild type plastome copies were detected. No spontaneous mutants were obtained. The transformation efficiency is similar to that of tobacco plastids. All transplastomic T0 plants were fertile and T1 progeny was uniformly spectinomycin resistant, showing the stability of the plastid transgene. This is the first report on the generation of fertile transplastomic soybean.

Entities: Chemical Gene Species

Mesh：

Substances：
Spectinomycin

Year: 2004 PMID： 15604694 DOI： 10.1007/s11103-004-0192-4

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

32 in total

Review 1. Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products.

Authors: L Bogorad
Journal: Trends Biotechnol Date: 2000-06 Impact factor: 19.536

Review 2. Engineering the plastid genome of higher plants.

Authors: Pal Maliga
Journal: Curr Opin Plant Biol Date: 2002-04 Impact factor: 7.834

3. Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.

Authors: S Corneille; K Lutz; Z Svab; P Maliga
Journal: Plant J Date: 2001-07 Impact factor: 6.417

4. Stable transformation of plastids in higher plants.

Authors: Z Svab; P Hajdukiewicz; P Maliga
Journal: Proc Natl Acad Sci U S A Date: 1990-11 Impact factor: 11.205

5. Chloroplast transformation in oilseed rape.

Authors: Bing-Kai Hou; Yi-Hua Zhou; Li-Hong Wan; Zhong-Lin Zhang; Gui-Fang Shen; Zheng-Hua Chen; Zan-Min Hu
Journal: Transgenic Res Date: 2003-02 Impact factor: 2.788

Review 6. Plastid transformation in higher plants.

Authors: Pal Maliga
Journal: Annu Rev Plant Biol Date: 2004 Impact factor: 26.379

7. High-yield production of a human therapeutic protein in tobacco chloroplasts.

Authors: J M Staub; B Garcia; J Graves; P T Hajdukiewicz; P Hunter; N Nehra; V Paradkar; M Schlittler; J A Carroll; L Spatola; D Ward; G Ye; D A Russell
Journal: Nat Biotechnol Date: 2000-03 Impact factor: 54.908

8. Removal of antibiotic resistance genes from transgenic tobacco plastids.

Authors: S Iamtham; A Day
Journal: Nat Biotechnol Date: 2000-11 Impact factor: 54.908

9. Plastid inheritance in Pisum sativum L.

Authors: N O Polans; J L Corriveau; A W Coleman
Journal: Curr Genet Date: 1990-12 Impact factor: 3.886

10. Mutation proximal to the tRNA binding region of the Nicotiana plastid 16S rRNA confers resistance to spectinomycin.

Authors: Z Svab; P Maliga
Journal: Mol Gen Genet Date: 1991-08

52 in total

1. High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.

Authors: Vladimir T Valkov; Daniela Gargano; Carmela Manna; Gelsomina Formisano; Philip J Dix; John C Gray; Nunzia Scotti; Teodoro Cardi
Journal: Transgenic Res Date: 2010-05-13 Impact factor: 2.788

10. Stable expression of Gal/GalNAc lectin of Entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis.

Authors: Seethamahalakshmi Chebolu; Henry Daniell
Journal: Plant Biotechnol J Date: 2007-03 Impact factor: 9.803

Generation of fertile transplastomic soybean.

Review 1. Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products.

Review 2. Engineering the plastid genome of higher plants.

3. Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.

4. Stable transformation of plastids in higher plants.

5. Chloroplast transformation in oilseed rape.

Review 6. Plastid transformation in higher plants.

7. High-yield production of a human therapeutic protein in tobacco chloroplasts.

8. Removal of antibiotic resistance genes from transgenic tobacco plastids.

9. Plastid inheritance in Pisum sativum L.

10. Mutation proximal to the tRNA binding region of the Nicotiana plastid 16S rRNA confers resistance to spectinomycin.

1. High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.

2. Construction of a species-specific vector for improved plastid transformation efficiency in Capsicum annuum L.

3. Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin.

4. Complete chloroplast genome sequence of Gycine max and comparative analyses with other legume genomes.

Review 5. Breakthrough in chloroplast genetic engineering of agronomically important crops.

6. Stability of soybean recombinant plastome over six generations.

7. Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.

8. Genetic transformation of plastids of different Solanaceae species using tobacco cells as organelle hosts.

9. Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts.

10. Stable expression of Gal/GalNAc lectin of Entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis.