BACKGROUND: This study was conducted to evaluate whether tissue transglutaminase (tTG) may be involved in airway inflammation of toluene diisocyanate-induced occupational asthma (TDI-OA). METHODS: We enrolled 93 patients with TDI-OA, 177 asymptomatic exposed subjects, 43 patients with allergic asthma, and 70 unexposed normal controls. The prevalence of serum immunoglobulin G (IgG) to tTG in the TDI-OA group (20.2%) was significantly higher than that in the three other groups (P < 0.001). RESULTS: TDI-OA patients with serum IgG to tTG had significantly lower methacholine PC(20) values (P < 0.02) and significantly higher prevalence of specific immunoglobulin E to vapor type TDI-human serum albumin conjugate (P < 0.01; r(2) = 0.411, P < 0.05). TDI exposure could increase tTG activity via reactive oxygen species (ROS) production, which was found to cross-link with cytokeratin 19 on immunoblot analysis. CONCLUSION: Therefore, TDI exposure may activate tTG via ROS-mediated mechanism in the airway epithelium leading to persistent airway inflammation in TDI-OA patients.
BACKGROUND: This study was conducted to evaluate whether tissue transglutaminase (tTG) may be involved in airway inflammation of toluene diisocyanate-induced occupational asthma (TDI-OA). METHODS: We enrolled 93 patients with TDI-OA, 177 asymptomatic exposed subjects, 43 patients with allergic asthma, and 70 unexposed normal controls. The prevalence of serum immunoglobulin G (IgG) to tTG in the TDI-OA group (20.2%) was significantly higher than that in the three other groups (P < 0.001). RESULTS:TDI-OA patients with serum IgG to tTG had significantly lower methacholine PC(20) values (P < 0.02) and significantly higher prevalence of specific immunoglobulin E to vapor type TDI-human serum albumin conjugate (P < 0.01; r(2) = 0.411, P < 0.05). TDI exposure could increase tTG activity via reactive oxygen species (ROS) production, which was found to cross-link with cytokeratin 19 on immunoblot analysis. CONCLUSION: Therefore, TDI exposure may activate tTG via ROS-mediated mechanism in the airway epithelium leading to persistent airway inflammation in TDI-OA patients.
Authors: Cristina E Mapp; Anthony A Fryer; Nicoletta De Marzo; Valeria Pozzato; Michele Padoan; Piera Boschetto; Richard C Strange; Anja Hemmingsen; Monica A Spiteri Journal: J Allergy Clin Immunol Date: 2002-05 Impact factor: 10.793
Authors: Berran Yucesoy; Victor J Johnson; Zana L Lummus; Grace E Kissling; Kara Fluharty; Denyse Gautrin; Jean-Luc Malo; André Cartier; Louis-Philippe Boulet; Joaquin Sastre; Santiago Quirce; Dori R Germolec; Susan M Tarlo; Maria-Jesus Cruz; Xavier Munoz; Michael I Luster; David I Bernstein Journal: Toxicol Sci Date: 2012-05-17 Impact factor: 4.849
Authors: Seoung Ju Park; Kyung Sun Lee; So Ri Kim; Han Jung Chae; Wan Hee Yoo; Dong Im Kim; Myoung Shin Jeon; Yong Chul Lee Journal: Inflamm Res Date: 2012-06-13 Impact factor: 4.575
Authors: Berran Yucesoy; Grace E Kissling; Victor J Johnson; Zana L Lummus; Denyse Gautrin; André Cartier; Louis-Philippe Boulet; Joaquin Sastre; Santiago Quirce; Susan M Tarlo; Maria-Jesus Cruz; Xavier Munoz; Michael I Luster; David I Bernstein Journal: J Occup Environ Med Date: 2015-12 Impact factor: 2.162