Wheat-alien metaphase I pairing of individual wheat genomes and D genome chromosomes in interspecific hybrids between Triticum aestivum L. and Aegilops geniculata Roth.

Homoeologous metaphase I (MI) pairing of Triticum aestivum x Aegilops geniculata hybrids (2n = 5x = 35, ABDU(g)M(g)) has been examined by an in situ hybridization procedure permitting simultaneous discrimination of A, B, D and wild genomes. The seven D genome chromosomes (and their arms, except for 6D and 7D) plus some additional wheat chromosomes were also identified. Wheat-wild MI associations represented more than 60% of total, with an average ratio of 5:1:12 for those involving the A, B and D genomes, respectively. A remarkable between-chromosome variation for the level of wheat-wild genetic exchange is expected within each wheat genome. However, it can be concluded that 3DL and 5DL are the crop genome locations with the highest probability of being transferred to Ae. geniculata. Hybrids derived from the ph2b wheat mutant line showed increased MI pairing but identical pattern of homoeologous associations than those with active Ph2.