| Literature DB >> 19546004 |
Takashi Sakamoto1, Masa-aki Inoue, Akio Ojida, Itaru Hamachi.
Abstract
We developed a new fluorescence assay system for GSK3beta-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.Entities:
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Year: 2009 PMID： 19546004 DOI： 10.1016/j.bmcl.2009.05.121
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823