Literature DB >> 19542333

Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.

Devin Gillaspie1, Izabella Perkins, Kellie Larsen, Amy McCord, Scott Pangonis, Debra Sweger, Mohamed N Seleem, Nammalwar Sriranganathan, Burt E Anderson.   

Abstract

Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.

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Year:  2009        PMID： 19542333      PMCID： PMC2725459          DOI： 10.1128/AEM.00949-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  HMEC-1: establishment of an immortalized human microvascular endothelial cell line.

Authors:  E W Ades; F J Candal; R A Swerlick; V G George; S Summers; D C Bosse; T J Lawley
Journal:  J Invest Dermatol       Date:  1992-12       Impact factor: 8.551

2.  Improved expression vector for Brucella species.

Authors:  Mohamed N Seleem; Ramesh Vemulapalli; Stephen M Boyle; Gerhardt G Schurig; Nammalwar Sriranganathan
Journal:  Biotechniques       Date:  2004-11       Impact factor: 1.993

3.  Development and use of a conditional antisense mutagenesis system in mycobacteria.

Authors:  T Parish; N G Stoker
Journal:  FEMS Microbiol Lett       Date:  1997-09-01       Impact factor: 2.742

4.  Inducible antisense RNA expression in the characterization of gene functions in Streptococcus mutans.

Authors:  Bing Wang; Howard K Kuramitsu
Journal:  Infect Immun       Date:  2005-06       Impact factor: 3.441

5.  Autocrine role for interleukin-8 in Bartonella henselae-induced angiogenesis.

Authors:  Amy M McCord; Sandra I Resto-Ruiz; Burt E Anderson
Journal:  Infect Immun       Date:  2006-09       Impact factor: 3.441

6.  Antisense RNA modulation of alkyl hydroperoxide reductase levels in Helicobacter pylori correlates with organic peroxide toxicity but not infectivity.

Authors:  Matthew A Croxen; Peter B Ernst; Paul S Hoffman
Journal:  J Bacteriol       Date:  2007-03-02       Impact factor: 3.490

7.  A SacB mutagenesis strategy reveals that the Bartonella quintana variably expressed outer membrane proteins are required for bloodstream infection of the host.

Authors:  Joanna K MacKichan; Helen L Gerns; Yu-Ting Chen; Peng Zhang; Jane E Koehler
Journal:  Infect Immun       Date:  2007-12-10       Impact factor: 3.441

8.  Borrelia burgdorferi ftsZ plays a role in cell division.

Authors:  Lydia Dubytska; Henry P Godfrey; Felipe C Cabello
Journal:  J Bacteriol       Date:  2006-03       Impact factor: 3.490

9.  Characterization of a novel Rochalimaea species, R. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient.

Authors:  R L Regnery; B E Anderson; J E Clarridge; M C Rodriguez-Barradas; D C Jones; J H Carr
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948

Review 10.  Bartonella quintana characteristics and clinical management.

Authors:  Cédric Foucault; Philippe Brouqui; Didier Raoult
Journal:  Emerg Infect Dis       Date:  2006-02       Impact factor: 6.883
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  11 in total

1.  The BatR/BatS two-component regulatory system controls the adaptive response of Bartonella henselae during human endothelial cell infection.

Authors:  Maxime Quebatte; Michaela Dehio; David Tropel; Andrea Basler; Isabella Toller; Guenter Raddatz; Philipp Engel; Sonja Huser; Hermine Schein; Hillevi L Lindroos; Siv G E Andersson; Christoph Dehio
Journal:  J Bacteriol       Date:  2010-04-23       Impact factor: 3.490

2.  Zebrafish embryo model of Bartonella henselae infection.

Authors:  Amorce Lima; Byeong J Cha; Jahanshah Amin; Lisa K Smith; Burt Anderson
Journal:  Zebrafish       Date:  2014-07-15       Impact factor: 1.985

3.  Characterization of the general stress response in Bartonella henselae.

Authors:  Nhan Tu; Amorce Lima; Zahra Bandeali; Burt Anderson
Journal:  Microb Pathog       Date:  2015-12-25       Impact factor: 3.738

Review 4.  CRISPR and Antisense RNA Technology: Exploiting Nature's Tool to Restrain Virulence in Tenacious Pathogens.

Authors:  Priyanka Ashwath; Disha Somanath; Akhila Dharnappa Sannejal
Journal:  Mol Biotechnol       Date:  2022-08-18       Impact factor: 2.860

5.  Heme degrading protein HemS is involved in oxidative stress response of Bartonella henselae.

Authors:  MaFeng Liu; Henri-Jean Boulouis; Francis Biville
Journal:  PLoS One       Date:  2012-05-31       Impact factor: 3.240

6.  Heme binding proteins of Bartonella henselae are required when undergoing oxidative stress during cell and flea invasion.

Authors:  MaFeng Liu; Yann Ferrandez; Emilie Bouhsira; Martine Monteil; Michel Franc; Henri-Jean Boulouis; Francis Biville
Journal:  PLoS One       Date:  2012-10-29       Impact factor: 3.240

7.  Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown.

Authors:  MaFeng Liu; MengYi Wang; DeKang Zhu; MingShu Wang; RenYong Jia; Shun Chen; KunFeng Sun; Qiao Yang; Ying Wu; XiaoYue Chen; Francis Biville; AnChun Cheng
Journal:  Sci Rep       Date:  2016-11-15       Impact factor: 4.379

8.  A family of genus-specific RNAs in tandem with DNA-binding proteins control expression of the badA major virulence factor gene in Bartonella henselae.

Authors:  Nhan Tu; Ronan K Carroll; Andy Weiss; Lindsey N Shaw; Gael Nicolas; Sarah Thomas; Amorce Lima; Udoka Okaro; Burt Anderson
Journal:  Microbiologyopen       Date:  2016-10-28       Impact factor: 3.139

9.  A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a.

Authors:  Isaac Gifford; Summer Vance; Giang Nguyen; Alison M Berry
Journal:  Front Microbiol       Date:  2019-09-24       Impact factor: 5.640

10.  Bartonella quintana deploys host and vector temperature-specific transcriptomes.

Authors:  Stephanie Abromaitis; Christopher S Nelson; Domenic Previte; Kyong S Yoon; J Marshall Clark; Joseph L DeRisi; Jane E Koehler
Journal:  PLoS One       Date:  2013-03-12       Impact factor: 3.240
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