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Literature DB >> 19542329

Alleviation of proteolytic sensitivity to enhance recombinant lipase production in Escherichia coli.

Abstract

Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.

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Year: 2009 PMID： 19542329 PMCID： PMC2725454 DOI： 10.1128/AEM.00740-09

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

17 in total

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Journal: Structure Date: 1994-04-15 Impact factor: 5.006

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Authors: Yali Xu; Amrita Yasin; Raymond Tang; Jeno M Scharer; Murray Moo-Young; C Perry Chou
Journal: Appl Microbiol Biotechnol Date: 2008-08-29 Impact factor: 4.813

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Authors: Wen-Chen Suen; Ningyan Zhang; Li Xiao; Vincent Madison; Aleksey Zaks
Journal: Protein Eng Des Sel Date: 2004-01-12 Impact factor: 1.650

6 in total

1. Development of a homologous expression system for and systematic site-directed mutagenesis analysis of thurincin H, a bacteriocin produced by Bacillus thuringiensis SF361.

Authors: Gaoyan Wang; David C Manns; John J Churey; Randy W Worobo
Journal: Appl Environ Microbiol Date: 2014-06 Impact factor: 4.792

2. New efficient recombinant expression system to engineer Candida antarctica lipase B.

Authors: Stéphane Emond; Cédric Montanier; Jean-Marc Nicaud; Alain Marty; Pierre Monsan; Isabelle André; Magali Remaud-Siméon
Journal: Appl Environ Microbiol Date: 2010-02-19 Impact factor: 4.792

3. Developing an extended genomic engineering approach based on recombineering to knock-in heterologous genes to Escherichia coli genome.

Authors: Karan Sukhija; Michael Pyne; Saad Ali; Valerie Orr; Daryoush Abedi; Murray Moo-Young; C Perry Chou
Journal: Mol Biotechnol Date: 2012-06 Impact factor: 2.695