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Literature DB >> 19540882

A generally applicable assay for the quantification of inhibitory effects on PCR.

Salome Schneider¹, Jürg Enkerli, Franco Widmer.

Abstract

PCR amplification of target genes from environmental DNA extracts can suffer from PCR inhibition, caused by co-extracted substances. No simple assay has been available to quantify this inhibition. Therefore, a generally applicable PCR inhibition-assay was developed, which allows determination of statistically significant inhibition of PCR. This information is important when documenting quality of DNA in environmental extracts used as template for PCR.

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Year: 2009 PMID： 19540882 DOI： 10.1016/j.mimet.2009.06.010

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

6 in total

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Authors: Nathalie Morel; Hervé Volland; Julie Dano; Patricia Lamourette; Patricia Sylvestre; Michèle Mock; Christophe Créminon
Journal: Appl Environ Microbiol Date: 2012-07-06 Impact factor: 4.792

2. Chromosome-Directed PCR-Based Detection and Quantification of Bacillus cereus Group Members with Focus on B. thuringiensis Serovar israelensis Active against Nematoceran Larvae.

Authors: Salome Schneider; Niels B Hendriksen; Petter Melin; Jan O Lundström; Ingvar Sundh
Journal: Appl Environ Microbiol Date: 2015-05-15 Impact factor: 4.792

3. Do Multi-year Applications of Bacillus thuringiensis subsp. israelensis for Control of Mosquito Larvae Affect the Abundance of B. cereus Group Populations in Riparian Wetland Soils?

Authors: Salome Schneider; Tania Tajrin; Jan O Lundström; Niels B Hendriksen; Petter Melin; Ingvar Sundh
Journal: Microb Ecol Date: 2017-06-10 Impact factor: 4.552

A generally applicable assay for the quantification of inhibitory effects on PCR.

1. Fast and sensitive detection of Bacillus anthracis spores by immunoassay.

2. Chromosome-Directed PCR-Based Detection and Quantification of Bacillus cereus Group Members with Focus on B. thuringiensis Serovar israelensis Active against Nematoceran Larvae.

3. Do Multi-year Applications of Bacillus thuringiensis subsp. israelensis for Control of Mosquito Larvae Affect the Abundance of B. cereus Group Populations in Riparian Wetland Soils?

4. Microbiological quality of tempeh with different wraps: banana leaf versus plastic.

5. Improving eDNA yield and inhibitor reduction through increased water volumes and multi-filter isolation techniques.

6. A localized PCR inhibitor in a porcelain crab suggests a protective role.