Weronika Krzeszowiec1, Halina Gabryś. 1. Department of Plant Physiology and Biochemistry; Faculty of Biochemistry, Biophysics and Biotechnology; Jagiellonian University; Kraków, Poland.
Abstract
BACKGROUND: The mechanism of the light-dependent movements of chloroplasts is based on actin and myosin but its details are largely unknown. The movements are activated by blue light in terrestrial angiosperms. The aim of the present study was to determine the role of myosin associated with the chloroplast surface in the light-induced chloroplast responses in Arabidopsis thaliana. The localization of myosins was investigated under blue light intensities generating avoidance and accumulation responses of chloroplasts. The localization was compared in wild type plants and in phot2 mutant lacking the avoidance response. RESULTS: Wild type and phot2 mutant plants were irradiated with strong (36 microEm(-2)s(-1)) and/or weak (0.8 microEm(-2)s(-1)) blue light. The leaf tissue was immunolabeled with antimyosin antibodies. Different arrangements of myosins were observed in the mesophyll depending on the fluence rate in wild type plants. In tissue irradiated with weak blue light myosins were associated with chloroplast envelopes. In contrast, in tissue irradiated with strong blue light chloroplasts were almost myosin-free. The effect did not occur in red light and in the phot2 mutant. CONCLUSIONS: Myosin displacement is blue light specific, i.e., it is associated with the activation of a specific blue-light photoreceptor. We suggest that the reorganization of myosins is essential for chloroplast movement. Myosins appear to be the final step of the signal transduction pathway starting with phototropin2 and leading to chloroplast movements.
BACKGROUND: The mechanism of the light-dependent movements of chloroplasts is based on actin and myosin but its details are largely unknown. The movements are activated by blue light in terrestrial angiosperms. The aim of the present study was to determine the role of myosin associated with the chloroplast surface in the light-induced chloroplast responses in Arabidopsis thaliana. The localization of myosins was investigated under blue light intensities generating avoidance and accumulation responses of chloroplasts. The localization was compared in wild type plants and in phot2 mutant lacking the avoidance response. RESULTS: Wild type and phot2 mutant plants were irradiated with strong (36 microEm(-2)s(-1)) and/or weak (0.8 microEm(-2)s(-1)) blue light. The leaf tissue was immunolabeled with antimyosin antibodies. Different arrangements of myosins were observed in the mesophyll depending on the fluence rate in wild type plants. In tissue irradiated with weak blue light myosins were associated with chloroplast envelopes. In contrast, in tissue irradiated with strong blue light chloroplasts were almost myosin-free. The effect did not occur in red light and in the phot2 mutant. CONCLUSIONS:Myosin displacement is blue light specific, i.e., it is associated with the activation of a specific blue-light photoreceptor. We suggest that the reorganization of myosins is essential for chloroplast movement. Myosins appear to be the final step of the signal transduction pathway starting with phototropin2 and leading to chloroplast movements.
Entities:
Keywords:
Arabidopsis; blue light; chloroplast movements; myosins; phototropins
Authors: T Sakai; T Kagawa; M Kasahara; T E Swartz; J M Christie; W R Briggs; M Wada; K Okada Journal: Proc Natl Acad Sci U S A Date: 2001-05-22 Impact factor: 11.205