INTRODUCTION: Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. METHODS: Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. RESULTS: In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p < 0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. DISCUSSION AND CONCLUSION: These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.
INTRODUCTION: Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. METHODS:Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. RESULTS: In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p < 0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. DISCUSSION AND CONCLUSION: These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.
Authors: Reina Haque; D N Guha Mazumder; Sambit Samanta; Nilima Ghosh; David Kalman; Meera M Smith; Soma Mitra; Amal Santra; Sarbari Lahiri; Subhankar Das; Binay K De; Allan H Smith Journal: Epidemiology Date: 2003-03 Impact factor: 4.822
Authors: Angeline S Andrew; Amy J Warren; Aaron Barchowsky; Kaili A Temple; Linda Klei; Nicole V Soucy; Kimberley A O'Hara; Joshua W Hamilton Journal: Environ Health Perspect Date: 2003-05 Impact factor: 9.031
Authors: Christopher D Heaney; Brittany Kmush; Ana Navas-Acien; Kevin Francesconi; Walter Gössler; Kerry Schulze; DeLisa Fairweather; Sucheta Mehra; Kenrad E Nelson; Sabra L Klein; Wei Li; Hasmot Ali; Saijuddin Shaikh; Rebecca D Merrill; Lee Wu; Keith P West; Parul Christian; Alain B Labrique Journal: Environ Res Date: 2015-07-15 Impact factor: 6.498
Authors: Barrett M Welch; Adam Branscum; Sharia M Ahmed; Perry Hystad; Ellen Smit; Sakila Afroz; Meghan Megowan; Mostofa Golam; Md Omar Sharif Ibne Hasan; Mohammad L Rahman; Quazi Quamruzzaman; David C Christiani; Molly L Kile Journal: Environ Int Date: 2019-04-30 Impact factor: 9.621