ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants belonging to the family Polygonaceae in Chinese pharmacopoeia possess important medicinal efficacy in traditional Chinese medicines. AIM OF THE STUDY: DNA barcodes are first used to discriminate the Polygonaceae in Chinese pharmacopoeia and their adulterants. MATERIALS AND METHODS: DNA samples, extracted from thirty-eight specimens belonging to eighteen species in Polygonaceae, were used as templates. Eight candidate barcodes were amplified by polymerase chain reaction. Sequence analysis was accomplished by CodonCode Aligner V 2.06 and DNAman V 6. Species identification was performed using MEGA V 4.0. RESULTS: The amplification efficiency of six candidate DNA barcodes (rbcL, trnH-psbA, ndhJ, rpoB, rpoC1, accD) was 100%, while the efficiency of YCF5 and nrITS was 56% and 44%, respectively. The interspecific divergence was highest for the trnH-psbA (20.05%), followed by the nrITS (14.01%) across all species pairs, while intraspecific variation both within populations and between populations was absent (0.0%). The trnH-psbA can not only distinguish ten species of Polygonaceae in Chinese pharmacopoeia, but also recognize eight other species of Polygonaceae including their adulterants. CONCLUSION: Our findings show that DNA barcoding is an efficient tool for identification of Polygonaceae in Chinese pharmacopoeia and their adulterants.
ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants belonging to the family Polygonaceae in Chinese pharmacopoeia possess important medicinal efficacy in traditional Chinese medicines. AIM OF THE STUDY: DNA barcodes are first used to discriminate the Polygonaceae in Chinese pharmacopoeia and their adulterants. MATERIALS AND METHODS: DNA samples, extracted from thirty-eight specimens belonging to eighteen species in Polygonaceae, were used as templates. Eight candidate barcodes were amplified by polymerase chain reaction. Sequence analysis was accomplished by CodonCode Aligner V 2.06 and DNAman V 6. Species identification was performed using MEGA V 4.0. RESULTS: The amplification efficiency of six candidate DNA barcodes (rbcL, trnH-psbA, ndhJ, rpoB, rpoC1, accD) was 100%, while the efficiency of YCF5 and nrITS was 56% and 44%, respectively. The interspecific divergence was highest for the trnH-psbA (20.05%), followed by the nrITS (14.01%) across all species pairs, while intraspecific variation both within populations and between populations was absent (0.0%). The trnH-psbA can not only distinguish ten species of Polygonaceae in Chinese pharmacopoeia, but also recognize eight other species of Polygonaceae including their adulterants. CONCLUSION: Our findings show that DNA barcoding is an efficient tool for identification of Polygonaceae in Chinese pharmacopoeia and their adulterants.
Authors: Ramanujam Srirama; J U Santhosh Kumar; G S Seethapathy; Steven G Newmaster; S Ragupathy; K N Ganeshaiah; R Uma Shaanker; Gudasalamani Ravikanth Journal: Drug Saf Date: 2017-08 Impact factor: 5.606
Authors: M Ajmal Ali; Gábor Gyulai; Norbert Hidvégi; Balázs Kerti; Fahad M A Al Hemaid; Arun K Pandey; Joongku Lee Journal: Saudi J Biol Sci Date: 2014-03-31 Impact factor: 4.219
Authors: Octavian Tudorel Olaru; Luanne Venables; Maryna VAN DE Venter; George Mihai Nitulescu; Denisa Margina; Demetrios A Spandidos; Aristidis M Tsatsakis Journal: Oncol Lett Date: 2015-07-02 Impact factor: 2.967