| Literature DB >> 19504065 |
Jean Cozzi1, Ignacio Anegon, Valérie Braun, Anne-Catherine Gross, Christel Merrouche, Yacine Cherifi.
Abstract
In the absence of germ-line-competent ES cells in the rat, pronuclear microinjection remains an essential tool to generate transgenic rat models. However, DNA microinjection procedures first developed for mouse do not provide scientists with satisfying results when applied to rat. Here we describe optimized procedures for rat with a special focus on rat embryo production, in vitro incubation and culture, DNA pronuclear injection, and the transfer of embryos into foster females.Entities:
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Year: 2009 PMID： 19504065 DOI： 10.1007/978-1-60327-019-9_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745