Literature DB >> 19502439

Structure-based rational design of a phosphotriesterase.

Colin J Jackson1, Kahli Weir, Anthony Herlt, Jeevan Khurana, Tara D Sutherland, Irene Horne, Christopher Easton, Robyn J Russell, Colin Scott, John G Oakeshott.   

Abstract

In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.

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Year:  2009        PMID： 19502439      PMCID： PMC2725513          DOI： 10.1128/AEM.00629-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Structural determinants of the substrate and stereochemical specificity of phosphotriesterase.

Authors:  M Chen-Goodspeed; M A Sogorb; F Wu; S B Hong; F M Raushel
Journal:  Biochemistry       Date:  2001-02-06       Impact factor: 3.162

2.  Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents.

Authors:  Catherine Mee-Hie Cho; Ashok Mulchandani; Wilfred Chen
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

3.  The effects of substrate orientation on the mechanism of a phosphotriesterase.

Authors:  Colin J Jackson; Jian-Wei Liu; Michelle L Coote; David L Ollis
Journal:  Org Biomol Chem       Date:  2005-11-16       Impact factor: 3.876

4.  The enzymatic basis for pesticide bioremediation.

Authors:  Colin Scott; Gunjan Pandey; Carol J Hartley; Colin J Jackson; Matthew J Cheesman; Matthew C Taylor; Rinku Pandey; Jeevan L Khurana; Mark Teese; Chris W Coppin; Kahli M Weir; Rakesh K Jain; Rup Lal; Robyn J Russell; John G Oakeshott
Journal:  Indian J Microbiol       Date:  2008-05-01       Impact factor: 2.461

5.  Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site.

Authors:  Colin J Jackson; Paul D Carr; Hye-Kyung Kim; Jian-Wei Liu; Paul Herrald; Natasa Mitić; Gerhard Schenk; Clyde A Smith; David L Ollis
Journal:  Biochem J       Date:  2006-08-01       Impact factor: 3.857

6.  Evolution of an organophosphate-degrading enzyme: a comparison of natural and directed evolution.

Authors:  H Yang; P D Carr; S Yu McLoughlin; J W Liu; I Horne; X Qiu; C M J Jeffries; R J Russell; J G Oakeshott; D L Ollis
Journal:  Protein Eng       Date:  2003-02

7.  Metal-substrate interactions facilitate the catalytic activity of the bacterial phosphotriesterase.

Authors:  S B Hong; F M Raushel
Journal:  Biochemistry       Date:  1996-08-20       Impact factor: 3.162

8.  Purification and properties of the phosphotriesterase from Pseudomonas diminuta.

Authors:  D P Dumas; S R Caldwell; J R Wild; F M Raushel
Journal:  J Biol Chem       Date:  1989-11-25       Impact factor: 5.157

9.  Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos.

Authors:  Catherine Mee-Hie Cho; Ashok Mulchandani; Wilfred Chen
Journal:  Appl Environ Microbiol       Date:  2004-08       Impact factor: 4.792

10.  OpdA, a bacterial organophosphorus hydrolase, prevents lethality in rats after poisoning with highly toxic organophosphorus pesticides.

Authors:  Steven B Bird; Tara D Sutherland; Chip Gresham; John Oakeshott; Colin Scott; Michael Eddleston
Journal:  Toxicology       Date:  2008-03-10       Impact factor: 4.221
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  10 in total

1.  Pharmacokinetics of OpdA, an organophosphorus hydrolase, in the African green monkey.

Authors:  Colin J Jackson; Colin Scott; Angela Carville; Keith Mansfield; David L Ollis; Steven B Bird
Journal:  Biochem Pharmacol       Date:  2010-06-23       Impact factor: 5.858

2.  300-Fold increase in production of the Zn2+-dependent dechlorinase TrzN in soluble form via apoenzyme stabilization.

Authors:  Colin J Jackson; Christopher W Coppin; Paul D Carr; Alexey Aleksandrov; Matthew Wilding; Elena Sugrue; Joanna Ubels; Michael Paks; Janet Newman; Thomas S Peat; Robyn J Russell; Martin Field; Martin Weik; John G Oakeshott; Colin Scott
Journal:  Appl Environ Microbiol       Date:  2014-04-25       Impact factor: 4.792

3.  Use of OpdA, an organophosphorus (OP) hydrolase, prevents lethality in an African green monkey model of acute OP poisoning.

Authors:  Colin J Jackson; Angela Carville; Jeanine Ward; Keith Mansfield; David L Ollis; Tejvir Khurana; Steven B Bird
Journal:  Toxicology       Date:  2014-01-18       Impact factor: 4.221

4.  Kinetics and efficacy of an organophosphorus hydrolase in a rodent model of methyl-parathion poisoning.

Authors:  Chip Gresham; Christopher Rosenbaum; Romolo J Gaspari; Colin J Jackson; Steven B Bird
Journal:  Acad Emerg Med       Date:  2010-07       Impact factor: 3.451

5.  A 5000-fold increase in the specificity of a bacterial phosphotriesterase for malathion through combinatorial active site mutagenesis.

Authors:  Tatheer Naqvi; Andrew C Warden; Nigel French; Elena Sugrue; Paul D Carr; Colin J Jackson; Colin Scott
Journal:  PLoS One       Date:  2014-04-10       Impact factor: 3.240

Review 6.  Current and emerging strategies for organophosphate decontamination: special focus on hyperstable enzymes.

Authors:  Pauline Jacquet; David Daudé; Janek Bzdrenga; Patrick Masson; Mikael Elias; Eric Chabrière
Journal:  Environ Sci Pollut Res Int       Date:  2016-02-02       Impact factor: 4.223

Review 7.  Mining electron density for functionally relevant protein polysterism in crystal structures.

Authors:  James S Fraser; Colin J Jackson
Journal:  Cell Mol Life Sci       Date:  2010-12-29       Impact factor: 9.261

8.  The evolution of new enzyme function: lessons from xenobiotic metabolizing bacteria versus insecticide-resistant insects.

Authors:  Robyn J Russell; Colin Scott; Colin J Jackson; Rinku Pandey; Gunjan Pandey; Matthew C Taylor; Christopher W Coppin; Jian-Wei Liu; John G Oakeshott
Journal:  Evol Appl       Date:  2011-03       Impact factor: 5.183

Review 9.  From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.

Authors:  Raushan Kumar Singh; Manish Kumar Tiwari; Ranjitha Singh; Jung-Kul Lee
Journal:  Int J Mol Sci       Date:  2013-01-10       Impact factor: 5.923

10.  Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning.

Authors:  Michael Eddleston; R Eddie Clutton; Matthew Taylor; Adrian Thompson; Franz Worek; Harald John; Horst Thiermann; Colin Scott
Journal:  Clin Toxicol (Phila)       Date:  2019-08-27       Impact factor: 4.467
  10 in total

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