Literature DB >> 19480635

Analysis of insoluble proteins.

Sarah Trimpin1, Bill Brizzard.   

Abstract

The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20-30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time-consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.

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Year:  2009        PMID： 19480635     DOI： 10.2144/000113168

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  7 in total

1.  Validation of membrane protein topology models by oxidative labeling and mass spectrometry.

Authors:  Yan Pan; Xiang Ruan; Miguel A Valvano; Lars Konermann
Journal:  J Am Soc Mass Spectrom       Date:  2012-03-13       Impact factor: 3.109

2.  Laserspray ionization (LSI) ion mobility spectrometry (IMS) mass spectrometry.

Authors:  Ellen Inutan; Sarah Trimpin
Journal:  J Am Soc Mass Spectrom       Date:  2010-04-03       Impact factor: 3.109

3.  RACK1/Asc1p, a ribosomal node in cellular signaling.

Authors:  Nicole Rachfall; Kerstin Schmitt; Susanne Bandau; Nadine Smolinski; Armin Ehrenreich; Oliver Valerius; Gerhard H Braus
Journal:  Mol Cell Proteomics       Date:  2012-10-15       Impact factor: 5.911

4.  Expression of a leptospiral leucine-rich repeat protein using a food-grade vector in Lactobacillus plantarum, as a strategy for vaccine delivery.

Authors:  Chattip Suphatpahirapol; Thu-Ha Nguyen; Yada Tansiri; Yodying Yingchutrakul; Sittiruk Roytrakul; Supachai Nitipan; Worawidh Wajjwalku; Dietmar Haltrich; Siriwan Prapong; Suttipun Keawsompong
Journal:  3 Biotech       Date:  2019-08-08       Impact factor: 2.406

5.  Laserspray ionization, a new method for protein analysis directly from tissue at atmospheric pressure with ultrahigh mass resolution and electron transfer dissociation.

Authors:  Ellen D Inutan; Alicia L Richards; James Wager-Miller; Ken Mackie; Charles N McEwen; Sarah Trimpin
Journal:  Mol Cell Proteomics       Date:  2010-09-20       Impact factor: 5.911

6.  Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics.

Authors:  Bin Zhao; Jingqian Huo; Ning Liu; Jinlin Zhang; Jingao Dong
Journal:  Toxins (Basel)       Date:  2018-01-12       Impact factor: 4.546

7.  Differential Phospho-Signatures in Blood Cells Identify LRRK2 G2019S Carriers in Parkinson's Disease.

Authors:  Alicia Garrido; Enrique Santamaría; Joaquín Fernández-Irigoyen; Marta Soto; Cristina Simonet; Manel Fernández; Donina Obiang; Eduardo Tolosa; María-José Martí; Shalini Padmanabhan; Cristina Malagelada; Mario Ezquerra; Rubén Fernández-Santiago
Journal:  Mov Disord       Date:  2022-01-20       Impact factor: 9.698
  7 in total

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