Literature DB >> 19478437

Crystallization and preliminary X-ray analysis of Escherichia coli RNase G.

Pengfei Fang1, Jing Wang, Xu Li, Min Guo, Li Xing, Xu Cao, Yi Zhu, Yan Gao, Liwen Niu, Maikun Teng.   

Abstract

The homologous RNases RNase E and RNase G are widely distributed in bacteria and function in many important physiological processes, including mRNA degradation, rRNA maturation and so on. In this study, the crystallization and preliminary X-ray analysis of RNase G from Escherichia coli is described. Purified recombinant E. coli RNase G, which has 497 amino acids, was crystallized in the cubic space group F432, with unit-cell parameters a = b = c = 219.84 A. X-ray diffraction data were collected to a resolution of 3.4 A.

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Year:  2009        PMID： 19478437      PMCID： PMC2688416          DOI： 10.1107/S1744309109015802

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

1.  Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA.

Authors:  M Wachi; G Umitsuki; M Shimizu; A Takada; K Nagai
Journal:  Biochem Biophys Res Commun       Date:  1999-06-07       Impact factor: 3.575

2.  RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA.

Authors:  Z Li; S Pandit; M P Deutscher
Journal:  EMBO J       Date:  1999-05-17       Impact factor: 11.598

3.  RNase G-dependent degradation of the eno mRNA encoding a glycolysis enzyme enolase in Escherichia coli.

Authors:  Naoko Kaga; Genryou Umitsuki; Kazuo Nagai; Masaaki Wachi
Journal:  Biosci Biotechnol Biochem       Date:  2002-10       Impact factor: 2.043

4.  Structure of Escherichia coli RNase E catalytic domain and implications for RNA turnover.

Authors:  Anastasia J Callaghan; Maria Jose Marcaida; Jonathan A Stead; Kenneth J McDowall; William G Scott; Ben F Luisi
Journal:  Nature       Date:  2005-10-20       Impact factor: 49.962

5.  RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton.

Authors:  Aziz Taghbalout; Lawrence Rothfield
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-22       Impact factor: 11.205

6.  Involvement of RNase G in in vivo mRNA metabolism in Escherichia coli.

Authors:  G Umitsuki; M Wachi; A Takada; T Hikichi; K Nagai
Journal:  Genes Cells       Date:  2001-05       Impact factor: 1.891

7.  Cytoplasmic axial filaments in Escherichia coli cells: possible function in the mechanism of chromosome segregation and cell division.

Authors:  Y Okada; M Wachi; A Hirata; K Suzuki; K Nagai; M Matsuhashi
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

8.  The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

Authors:  K J McDowall; R G Hernandez; S Lin-Chao; S N Cohen
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

9.  The phylogenetic distribution of bacterial ribonucleases.

Authors:  Ciarán Condon; Harald Putzer
Journal:  Nucleic Acids Res       Date:  2002-12-15       Impact factor: 16.971

10.  The quaternary structure of RNase G from Escherichia coli.

Authors:  Douglas J Briant; Janet S Hankins; Michael A Cook; George A Mackie
Journal:  Mol Microbiol       Date:  2003-11       Impact factor: 3.501
  10 in total

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