Literature DB >> 19478434

Cloning, purification, crystallization and preliminary crystallographic analysis of a ribokinase from Staphylococcus aureus.

Lin Wang1, Haipeng Wang, Jianbin Ruan, Changlin Tian, Baolin Sun, Jianye Zang.   

Abstract

The gene SA239 from Staphylococcus aureus encodes a ribokinase that catalyzes the phosphorylation of D-ribose to produce ribose-5-phosphate. Sa239 was crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.9 A resolution and belonged to space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 91.8, c = 160.7 A. Preliminary crystallographic analysis revealed that the Matthews coefficient V(M) was 3.01 A(3) Da(-1), indicating the presence of one molecule in the asymmetric unit.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19478434      PMCID： PMC2688413          DOI： 10.1107/S1744309109014833

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

2.  The significance of ribokinase for ribose utilization by Escherichia coli.

Authors:  A Anderson; R A Cooper
Journal:  Biochim Biophys Acta       Date:  1969-02-18

3.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

4.  Ribokinase from E. coli: expression, purification, and substrate specificity.

Authors:  Dmitry V Chuvikovsky; Roman S Esipov; Yuri S Skoblov; Larisa A Chupova; Tatyana I Muravyova; Anatoly I Miroshnikov; Seppo Lapinjoki; Igor A Mikhailopulo
Journal:  Bioorg Med Chem       Date:  2006-06-19       Impact factor: 3.641

5.  Activation of ribokinase by monovalent cations.

Authors:  C Evalena Andersson; Sherry L Mowbray
Journal:  J Mol Biol       Date:  2002-01-18       Impact factor: 5.469

6.  The effect of inorganic phosphate on the activity of bacterial ribokinase.

Authors:  M C Maj; R S Gupta
Journal:  J Protein Chem       Date:  2001-02

7.  Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8 A resolution: insights into a new family of kinase structures.

Authors:  J A Sigrell; A D Cameron; T A Jones; S L Mowbray
Journal:  Structure       Date:  1998-02-15       Impact factor: 5.006

8.  D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.

Authors:  J E Lopilato; J L Garwin; S D Emr; T J Silhavy; J R Beckwith
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

9.  Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases.

Authors:  P Bork; C Sander; A Valencia
Journal:  Protein Sci       Date:  1993-01       Impact factor: 6.725
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.