| Literature DB >> 1947428 |
M Debue1, P Gautier, C Hackel, A Van Elsen, A Herzog, G Bigaignon, A Bollen.
Abstract
Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.Entities:
Mesh：
Year: 1991 PMID： 1947428 DOI： 10.1016/0923-2508(91)90189-h
Source DB: PubMed Journal: Res Microbiol ISSN： 0923-2508 Impact factor: 3.992