OBJECTIVE: To study HLA class I and class II association in Tunisian patients with reactive (ReA) and undifferentiated arthritis (UA). METHODS: The study included 17 patients with ReA defined according to the European Spondylarthropathy Study Group criteria for spondylarthropathy (SpA), 11 patients classified as having undifferentiated arthritis and 100 unrelated healthy controls. HLA class I antigens were typed serologically and HLA class II alleles were genotyped molecularly by the polymerase chain reaction with sequence-specific primers technique. RESULTS: There was a major difference between HLA alleles in ReA and UA patients when compared separately with controls. Increased frequencies of HLA-B27 (p=7.76 10-12, OR=59.30), HLA-B51 (p=0.015, OR=4.91) and HLA-DRB1*04 (p=0.033, OR=2.90) alleles were found in patients with ReA but not in patients with UA. HLA-B27 was not expressed totally in our cohort of UA patients. A significant increase of HLA-B15 (p=0.002, OR=18.40) and a moderate increase of HLA-B7 (p=0.043, OR=5.15) was found in patients with UA, but not in patients with ReA. In the B27 negative patients, HLA-DRB1*04 association with ReA was found independently of B27. CONCLUSION: Our data confirmed a significant association of HLA-B27 with ReA in the Tunisian population. Our results also suggested that some of the additional HLA antigens were associated with ReA including HLA-B51 and HLA-DRB1*04 alleles. UA seemed to have a genetic background different from ReA in Tunisian patients.
OBJECTIVE: To study HLA class I and class II association in Tunisian patients with reactive (ReA) and undifferentiated arthritis (UA). METHODS: The study included 17 patients with ReA defined according to the European Spondylarthropathy Study Group criteria for spondylarthropathy (SpA), 11 patients classified as having undifferentiated arthritis and 100 unrelated healthy controls. HLA class I antigens were typed serologically and HLA class II alleles were genotyped molecularly by the polymerase chain reaction with sequence-specific primers technique. RESULTS: There was a major difference between HLA alleles in ReA and UApatients when compared separately with controls. Increased frequencies of HLA-B27 (p=7.76 10-12, OR=59.30), HLA-B51 (p=0.015, OR=4.91) and HLA-DRB1*04 (p=0.033, OR=2.90) alleles were found in patients with ReA but not in patients with UA. HLA-B27 was not expressed totally in our cohort of UApatients. A significant increase of HLA-B15 (p=0.002, OR=18.40) and a moderate increase of HLA-B7 (p=0.043, OR=5.15) was found in patients with UA, but not in patients with ReA. In the B27 negative patients, HLA-DRB1*04 association with ReA was found independently of B27. CONCLUSION: Our data confirmed a significant association of HLA-B27 with ReA in the Tunisian population. Our results also suggested that some of the additional HLA antigens were associated with ReA including HLA-B51 and HLA-DRB1*04 alleles. UA seemed to have a genetic background different from ReA in Tunisian patients.
Authors: Ana M Santos; Paola Peña; Mabel Avila; Ignacio Briceño; Carlos Jaramillo; Gilberto Vargas-Alarcon; Juan C Rueda; Eugenia-Lucia Saldarriaga; Jose-Ignacio Angarita; Nancy Martinez-Rodriguez; John Londono Journal: Clin Rheumatol Date: 2016-12-24 Impact factor: 2.980
Authors: John Londono; Ana Maria Santos; Paola Peña; Enrique Calvo; Luis R Espinosa; John D Reveille; Gilberto Vargas-Alarcon; Carlos A Jaramillo; Rafael Valle-Oñate; Mabel Avila; Consuelo Romero; Juan F Medina Journal: BMJ Open Date: 2015-11-11 Impact factor: 2.692
Authors: John Londono; Ana M Santos; Juan C Rueda; Enrique Calvo-Paramo; Ruben Burgos-Vargas; Gilberto Vargas-Alarcon; Nancy Martinez-Rodriguez; Sofia Arias-Correal; Guisselle-Nathalia Muñoz; Diana Padilla; Francy Cuervo; Viviana Reyes-Martinez; Santiago Bernal-Macías; Catalina Villota-Eraso; Luz M Avila-Portillo; Consuelo Romero; Juan F Medina Journal: RMD Open Date: 2020-09