BACKGROUND: We wanted to investigate whether heat shock protein (HSP) 27 and HSP 72 are induced in retinal ganglion cells (RGCs) after acute intraocular pressure (IOP)-induced ischaemia. METHODS: Retinal ischaemia was induced by acutely increasing IOP to 100-110 mmHg for 30 or 90 min unilaterally in Sprague Dawley rats. A fluorescent tracer (fluorogold, FG) was applied to the superior colliculi to label RGCs. Twenty-four hours, 1 week or 2 weeks after of IOP elevation, rats were killed, RGCs counted, and immunohistochemical labelling of the retina was performed. HSP-positive RGCs were counted and normalized HSP RGC counts determined. RESULTS: The ratio of FG-positive labelled RGCs in the experimental to the contralateral eye as a marker of RGC survival remained unchanged after 30 min of ischaemia: 1.09 +/- 0.11 at 1 week, and 0.94 +/- 0.28 at 2 weeks. After 90 min of ischaemia RGC survival decreased to 0.19 +/- 0.14 at 1 week, and 0.20 +/- 0.14 at 2 weeks. After 30 min of ischaemia, the normalized HSP 27- and HSP 72-positive RGC count was detected at highest levels (HSP 27: 5.42 +/- 1.18; HSP 72: 12.23 +/- 1.24) at 2 weeks compared with controls,whereas after 90 min ischaemia it was detected at higher levels at 1 week (HSP 27: 52.63 +/- 3.65; HSP 72: 206.84 +/- 60.38), as well as at 2 weeks (HSP 27: 89.00 +/- 17.21; HSP 72: 191.00 +/- 50.05). CONCLUSION: These results demonstrate an enhanced induction of HSP 27 and HSP 72 after 90 min acute IOP-induced ischaemia. In contrast to 30 min ischaemia, we showed time-dependent loss of RGCs after 90 min of ischaemia after 1 week or 2 weeks.
BACKGROUND: We wanted to investigate whether heat shock protein (HSP) 27 and HSP 72 are induced in retinal ganglion cells (RGCs) after acute intraocular pressure (IOP)-induced ischaemia. METHODS:Retinal ischaemia was induced by acutely increasing IOP to 100-110 mmHg for 30 or 90 min unilaterally in Sprague Dawley rats. A fluorescent tracer (fluorogold, FG) was applied to the superior colliculi to label RGCs. Twenty-four hours, 1 week or 2 weeks after of IOP elevation, rats were killed, RGCs counted, and immunohistochemical labelling of the retina was performed. HSP-positive RGCs were counted and normalized HSP RGC counts determined. RESULTS: The ratio of FG-positive labelled RGCs in the experimental to the contralateral eye as a marker of RGC survival remained unchanged after 30 min of ischaemia: 1.09 +/- 0.11 at 1 week, and 0.94 +/- 0.28 at 2 weeks. After 90 min of ischaemia RGC survival decreased to 0.19 +/- 0.14 at 1 week, and 0.20 +/- 0.14 at 2 weeks. After 30 min of ischaemia, the normalized HSP 27- and HSP 72-positive RGC count was detected at highest levels (HSP 27: 5.42 +/- 1.18; HSP 72: 12.23 +/- 1.24) at 2 weeks compared with controls,whereas after 90 min ischaemia it was detected at higher levels at 1 week (HSP 27: 52.63 +/- 3.65; HSP 72: 206.84 +/- 60.38), as well as at 2 weeks (HSP 27: 89.00 +/- 17.21; HSP 72: 191.00 +/- 50.05). CONCLUSION: These results demonstrate an enhanced induction of HSP 27 and HSP 72 after 90 min acute IOP-induced ischaemia. In contrast to 30 min ischaemia, we showed time-dependent loss of RGCs after 90 min of ischaemia after 1 week or 2 weeks.
Authors: Elad Moisseiev; Johnathon D Anderson; Sharon Oltjen; Mayank Goswami; Robert J Zawadzki; Jan A Nolta; Susanna S Park Journal: Curr Eye Res Date: 2017-06-21 Impact factor: 2.424
Authors: Michael R Olin; Brian M Andersen; David M Zellmer; Patrick T Grogan; Flavia E Popescu; Zhengming Xiong; Colleen L Forster; Charlie Seiler; Karen S SantaCruz; Wei Chen; Bruce R Blazar; John R Ohlfest Journal: Clin Cancer Res Date: 2010-09-21 Impact factor: 12.531
Authors: Vivian Rajeswaren; Jeffrey O Wong; Dana Yabroudi; Rooban B Nahomi; Johanna Rankenberg; Mi-Hyun Nam; Ram H Nagaraj Journal: Front Mol Biosci Date: 2022-04-11