BACKGROUND: We evaluated the degree of inflammatory response after ischemia/reperfusion injury by an extracorporeal normothermic autologous hemoperfusion of porcine livers. MATERIALS AND METHODS: Livers explanted from 7 pigs were perfused extracorporeally at 39 degrees C with autologous blood. Serum samples were obtained hourly until 6 hours from the beginning of reperfusion and assayed for 9 different cytokines. RESULTS: Significant elevations in interleukin 6 (IL-6) and IL-8 were noted following reperfusion (P < .001), with both demonstrating an increase which followed a sigmoid curve; other cytokines that were assessed showed no significant change. CONCLUSIONS: The ex vivo model excludes the liver from the influence of external systemic factors such as hormones, the autonomic nervous system, and other regulatory molecules produced elsewhere in the body, allowing the response to the ischemia/reperfusion injury to be studied in isolation and in considerable detail. Although this study examined a relatively short period, the increases in only IL-6 and IL-8 suggested that these are important molecules in the early phase after reperfusion.
BACKGROUND: We evaluated the degree of inflammatory response after ischemia/reperfusion injury by an extracorporeal normothermic autologous hemoperfusion of porcine livers. MATERIALS AND METHODS: Livers explanted from 7 pigs were perfused extracorporeally at 39 degrees C with autologous blood. Serum samples were obtained hourly until 6 hours from the beginning of reperfusion and assayed for 9 different cytokines. RESULTS: Significant elevations in interleukin 6 (IL-6) and IL-8 were noted following reperfusion (P < .001), with both demonstrating an increase which followed a sigmoid curve; other cytokines that were assessed showed no significant change. CONCLUSIONS: The ex vivo model excludes the liver from the influence of external systemic factors such as hormones, the autonomic nervous system, and other regulatory molecules produced elsewhere in the body, allowing the response to the ischemia/reperfusion injury to be studied in isolation and in considerable detail. Although this study examined a relatively short period, the increases in only IL-6 and IL-8 suggested that these are important molecules in the early phase after reperfusion.
Authors: Wen Yuan Chung; Amar M Eltweri; John Isherwood; Jonathan Haqq; Seok Ling Ong; Gianpiero Gravante; David M Lloyd; Matthew S Metcalfe; Ashley R Dennison Journal: J Vis Exp Date: 2013-12-18 Impact factor: 1.355
Authors: Gianpiero Gravante; Seok Ling Ong; Kevin West; Angus McGregor; Guy J Maddern; Matthew S Metcalfe; David M Lloyd; Ashley R Dennison Journal: Pathol Oncol Res Date: 2012-06-17 Impact factor: 3.201
Authors: Giuseppe Sconocchia; Giulia Lanzilli; Valeriana Cesarini; Domenico A Silvestris; Katayoun Rezvani; Roberto Arriga; Sara Caratelli; Ken Chen; Jinzhuang Dou; Carlo Cenciarelli; Gabriele Toietta; Silvia Baldari; Tommaso Sconocchia; Francesca De Paolis; Anna Aureli; Giandomenica Iezzi; Maria Irno Consalvo; Francesco Buccisano; Maria I Del Principe; Luca Maurillo; Adriano Venditti; Alessio Ottaviani; Giulio C Spagnoli Journal: Life Sci Alliance Date: 2022-10-14