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Literature DB >> 19449345

A facile method for reversibly linking a recombinant protein to DNA.

Russell P Goodman¹, Christoph M Erben, Jonathan Malo, Wei M Ho, Mireya L McKee, Achillefs N Kapanidis, Andrew J Turberfield.

Abstract

We present a facile method for linking recombinant proteins to DNA. It is based on the nickel-mediated interaction between a hexahistidine tag (His(6)-tag) and DNA functionalized with three nitrilotriacetic acid (NTA) groups. The resulting DNA-protein linkage is site-specific. It can be broken quickly and controllably by the addition of a chelating agent that binds nickel. We have used this new linker to bind proteins to a variety of DNA motifs commonly used in the fabrication of nanostructures by DNA self-assembly.

Entities: Chemical

Mesh：

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Year: 2009 PMID： 19449345 DOI： 10.1002/cbic.200900165

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

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Cited

14 in total

1. Covalent protein-oligonucleotide conjugates by copper-free click reaction.

Authors: Santoshkumar L Khatwani; Jun Sung Kang; Daniel G Mullen; Michael A Hast; Lorena S Beese; Mark D Distefano; T Andrew Taton
Journal: Bioorg Med Chem Date: 2012-05-17 Impact factor: 3.641

2. Template-directed covalent conjugation of DNA to native antibodies, transferrin and other metal-binding proteins.

Authors: Christian B Rosen; Anne L B Kodal; Jesper S Nielsen; David H Schaffert; Carsten Scavenius; Anders H Okholm; Niels V Voigt; Jan J Enghild; Jørgen Kjems; Thomas Tørring; Kurt V Gothelf
Journal: Nat Chem Date: 2014-07-20 Impact factor: 24.427

3. Acoustic force spectroscopy reveals subtle differences in cellulose unbinding behavior of carbohydrate-binding modules.

Authors: Markus Hackl; Edward V Contrada; Jonathan E Ash; Atharv Kulkarni; Jinho Yoon; Hyeon-Yeol Cho; Ki-Bum Lee; John M Yarbrough; Cesar A López; Sandrasegaram Gnanakaran; Shishir P S Chundawat
Journal: Proc Natl Acad Sci U S A Date: 2022-10-10 Impact factor: 12.779

A facile method for reversibly linking a recombinant protein to DNA.

1. Covalent protein-oligonucleotide conjugates by copper-free click reaction.

2. Template-directed covalent conjugation of DNA to native antibodies, transferrin and other metal-binding proteins.

3. Acoustic force spectroscopy reveals subtle differences in cellulose unbinding behavior of carbohydrate-binding modules.

4. Assessing histidine tags for recruiting deoxyribozymes to catalyze peptide and protein modification reactions.

5. Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.

Review 6. Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques.

Review 7. The Growing Development of DNA Nanostructures for Potential Healthcare-Related Applications.

8. Protein analysis by time-resolved measurements with an electro-switchable DNA chip.

9. Simple and efficient method to purify DNA-protein conjugates and its sensing applications.

10. DNA-mediated assembly of protein heterodimers on membrane surfaces.