OBJECTIVE: To study the inhibitive effect of extracts from Scindapsus aureus on alpha-glucosidase and its antioxidant activity. METHODS: The 70% ethanol extracts was extracted by petroleum, ethyl acetate and n-butanol, and the inhibitory activity against alpha-glucosidases and antioxidative effects of each fraction were determined in vitro, and the inhibitory kinetics of ethyl acetate fraction was investigated. RESULTS: The inhibitory activity of ethly acetate fraction against alpha-glucosidase was higher as its IC50 was 43.63 microg/mL. The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate fraction was a competitive type inhibitor, and the inhibition constants for free ennzyme (Ki) was determined to be 21.49 microg/mL. The antioxidative effects of ethly acetate and n-butanol fractions exhibited stronger than that of other fractions, the scavenging ability on DPPH at the concentration of 0.42 mg/mL were 88.5% and 93.6%. Ethly acetate fraction showed scavenging ability on autioxidantor, and the IC50 values was 0.27 mg/mL. CONCLUSION: Ethyld acetate fraction of extract from Scindapsus aureus has potential inhibitroy activity against alpha-glucosidase and antioxidant effects, and n-butanol fraction has great antioxidative effects.
OBJECTIVE: To study the inhibitive effect of extracts from Scindapsus aureus on alpha-glucosidase and its antioxidant activity. METHODS: The 70% ethanol extracts was extracted by petroleum, ethyl acetate and n-butanol, and the inhibitory activity against alpha-glucosidases and antioxidative effects of each fraction were determined in vitro, and the inhibitory kinetics of ethyl acetate fraction was investigated. RESULTS: The inhibitory activity of ethly acetate fraction against alpha-glucosidase was higher as its IC50 was 43.63 microg/mL. The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate fraction was a competitive type inhibitor, and the inhibition constants for free ennzyme (Ki) was determined to be 21.49 microg/mL. The antioxidative effects of ethly acetate and n-butanol fractions exhibited stronger than that of other fractions, the scavenging ability on DPPH at the concentration of 0.42 mg/mL were 88.5% and 93.6%. Ethly acetate fraction showed scavenging ability on autioxidantor, and the IC50 values was 0.27 mg/mL. CONCLUSION: Ethyld acetate fraction of extract from Scindapsus aureus has potential inhibitroy activity against alpha-glucosidase and antioxidant effects, and n-butanol fraction has great antioxidative effects.