UNLABELLED: We are developing a methodology for the noninvasive imaging of glucose transport in vivo with PET and (18)F-labeled 6-fluoro-6-deoxy-d-glucose ((18)F-6FDG), a tracer that is transported but not phosphorylated. To validate the method, we evaluated the biodistribution of (18)F-6FDG to test whether it is consistent with the known properties of glucose transport, particularly with regard to insulin stimulation of glucose transport. METHODS: Under glucose clamp conditions, rats were imaged at the baseline and under conditions of hyperinsulinemia. RESULTS: The images showed that the radioactivity concentration in skeletal muscle was higher in the presence of insulin than at the baseline. We also found evidence that the metabolism of (18)F-6FDG was negligible in several tissues. CONCLUSION: (18)F-6FDG is a valid tracer that can be used in in vivo transport studies. PET studies performed under glucose clamp conditions demonstrated that the uptake of nonphosphorylated glucose transport tracer (18)F-6FDG is sensitive to insulin stimulation.
UNLABELLED: We are developing a methodology for the noninvasive imaging of glucose transport in vivo with PET and (18)F-labeled 6-fluoro-6-deoxy-d-glucose ((18)F-6FDG), a tracer that is transported but not phosphorylated. To validate the method, we evaluated the biodistribution of (18)F-6FDG to test whether it is consistent with the known properties of glucose transport, particularly with regard to insulin stimulation of glucose transport. METHODS: Under glucose clamp conditions, rats were imaged at the baseline and under conditions of hyperinsulinemia. RESULTS: The images showed that the radioactivity concentration in skeletal muscle was higher in the presence of insulin than at the baseline. We also found evidence that the metabolism of (18)F-6FDG was negligible in several tissues. CONCLUSION: (18)F-6FDG is a valid tracer that can be used in in vivo transport studies. PET studies performed under glucose clamp conditions demonstrated that the uptake of nonphosphorylated glucose transport tracer (18)F-6FDG is sensitive to insulin stimulation.
Authors: Ali H Mokdad; Earl S Ford; Barbara A Bowman; William H Dietz; Frank Vinicor; Virginia S Bales; James S Marks Journal: JAMA Date: 2003-01-01 Impact factor: 56.272
Authors: L Sokoloff; M Reivich; C Kennedy; M H Des Rosiers; C S Patlak; K D Pettigrew; O Sakurada; M Shinohara Journal: J Neurochem Date: 1977-05 Impact factor: 5.372
Authors: D J Brooks; R P Beaney; A A Lammertsma; S Herold; D R Turton; S K Luthra; R S Frackowiak; D G Thomas; J Marshall; T Jones Journal: J Cereb Blood Flow Metab Date: 1986-04 Impact factor: 6.200
Authors: K Vyska; J R Magloire; C Freundlieb; A Höck; V Becker; A Schmid; L E Feinendegen; G Kloster; G Stöcklin; F J Schuier Journal: Eur J Nucl Med Date: 1985
Authors: Marc J Zuckerman; Ian S Menzies; Hoi Ho; Gavin G Gregory; Nancy A Casner; Roger S Crane; Jesus A Hernandez Journal: Dig Dis Sci Date: 2004-04 Impact factor: 3.199
Authors: D J Brooks; J S Gibbs; P Sharp; S Herold; D R Turton; S K Luthra; E M Kohner; S R Bloom; T Jones Journal: J Cereb Blood Flow Metab Date: 1986-04 Impact factor: 6.200
Authors: Bridgette A Christopher; Hsuan-Ming Huang; Jessica M Berthiaume; Tracy A McElfresh; Xiaoqin Chen; Colleen M Croniger; Raymond F Muzic; Margaret P Chandler Journal: Am J Physiol Heart Circ Physiol Date: 2010-09-17 Impact factor: 4.733
Authors: Charles A Kunos; Robert Debernardo; Jeffrey Fabien; Donald C Dobbins; Yuxia Zhang; James Brindle; Peter F Faulhaber Journal: J Nucl Med Radiat Ther Date: 2011-12-12
Authors: Hanne M M Rooijackers; Evita C Wiegers; Cees J Tack; Marinette van der Graaf; Bastiaan E de Galan Journal: Cell Mol Life Sci Date: 2015-10-31 Impact factor: 9.261