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Literature DB >> 19431307

The Molecular Weight and Aggregation of DNA.

L F Cavalieri, J F Deutsch, B H Rosenberg.

Abstract

The effects of enzymatic attack and of shear during the isolation and deproteinization of DNA have been investigated. Different methods of disaggregating DNA have been studied, and conditions under which reaggregation can occur are discussed. It was found that shaking with chloroform-octanol does not degrade DNA from the seven sources studied; that light scattering yields valid weight-average molecular weights for these samples; and that, when disaggregated, the molecular weights of these samples are in the range 1.2-2.4 million and the length-to-mass ratios are high.

Entities: Chemical

Year: 1961 PMID： 19431307 PMCID： PMC1366349 DOI： 10.1016/s0006-3495(61)86890-2

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

7 in total

The Molecular Weight and Aggregation of DNA.

1. A pH transition in the sedimentation behavior of deoxyribonucleic acid.

2. The influence of protein on heterogeneity of DNA.

3. A rapid method for preparing polymerized deoxyribonucleic acid from tissues, based on separation of nuclei.

4. The isolation of desoxyribonucleic acid from bacteriophages by an improved method.

5. STRAND SEPARATION AND SPECIFIC RECOMBINATION IN DEOXYRIBONUCLEIC ACIDS: PHYSICAL CHEMICAL STUDIES.

6. THE EFFECT OF HYDRODYNAMIC SHEAR ON THE DEOXYRIBONUCLEIC ACID FROM T(2) AND T(4) BACTERIOPHAGES.

7. THE REPLICATION OF DNA IN ESCHERICHIA COLI.

1. Physicochemical studies on the reaction between formaldehyde and DNA.

2. The renaturation of denatured DNA.

3. The replication of DNA II. The number of polynucleotide strands in the conserved unit of DNA.

4. The replication of DNA I. Two molecular classes of DNA.

5. The replication of DNA III. Changes in the number of strands in E. coli DNA during its replication cycle.

6. A DNA-acrylamide gel column for analyzing proteins that bind to DNA. I. DNA polymerase.

7. Evidence for allosterism in in vitro DNA synthesis on RNA templates.