Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Imaging transcription in living cells.

Literature DB >> 19416065

Imaging transcription in living cells.

Xavier Darzacq¹, Jie Yao, Daniel R Larson, Sébastien Z Causse, Lana Bosanac, Valeria de Turris, Vera M Ruda, Timothee Lionnet, Daniel Zenklusen, Benjamin Guglielmi, Robert Tjian, Robert H Singer.

Abstract

The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：
Transcription Factors

Year: 2009 PMID： 19416065 PMCID： PMC3166783 DOI： 10.1146/annurev.biophys.050708.133728

Source DB: PubMed Journal: Annu Rev Biophys ISSN： 1936-122X Impact factor: 12.981

97 in total

1. The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.

Authors: J G McNally; W G Müller; D Walker; R Wolford; G L Hager
Journal: Science Date: 2000-02-18 Impact factor: 47.728

2. FRAP reveals that mobility of oestrogen receptor-alpha is ligand- and proteasome-dependent.

Authors: D L Stenoien; K Patel; M G Mancini; M Dutertre; C L Smith; B W O'Malley; M A Mancini
Journal: Nat Cell Biol Date: 2001-01 Impact factor: 28.824

Review 3. Kinetic modelling approaches to in vivo imaging.

Authors: R D Phair; T Misteli
Journal: Nat Rev Mol Cell Biol Date: 2001-12 Impact factor: 94.444

4. Modulation of RNA polymerase assembly dynamics in transcriptional regulation.

Authors: Stanislaw A Gorski; Sara K Snyder; Sam John; Ingrid Grummt; Tom Misteli
Journal: Mol Cell Date: 2008-05-23 Impact factor: 17.970

5. Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei.

Authors: D A Jackson; F J Iborra; E M Manders; P R Cook
Journal: Mol Biol Cell Date: 1998-06 Impact factor: 4.138

6. Ligand-specific dynamics of the progesterone receptor in living cells and during chromatin remodeling in vitro.

Authors: Geetha V Rayasam; Cem Elbi; Dawn A Walker; Ronald Wolford; Terace M Fletcher; Dean P Edwards; Gordon L Hager
Journal: Mol Cell Biol Date: 2005-03 Impact factor: 4.272

7. RNA dynamics in live Escherichia coli cells.

Authors: Ido Golding; Edward C Cox
Journal: Proc Natl Acad Sci U S A Date: 2004-07-26 Impact factor: 11.205

8. P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo.

Authors: Zhuoyu Ni; Abbie Saunders; Nicholas J Fuda; Jie Yao; Jose-Ramon Suarez; Watt W Webb; John T Lis
Journal: Mol Cell Biol Date: 2007-12-10 Impact factor: 4.272

9. Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes.

Authors: Diana A Stavreva; Waltraud G Müller; Gordon L Hager; Carolyn L Smith; James G McNally
Journal: Mol Cell Biol Date: 2004-04 Impact factor: 4.272

10. Photobleaching reveals complex effects of inhibitors on transcribing RNA polymerase II in living cells.

Authors: Maud Fromaget; Peter R Cook
Journal: Exp Cell Res Date: 2007-05-18 Impact factor: 3.905

57 in total

1. Real-time observation of transcription initiation and elongation on an endogenous yeast gene.

Authors: Daniel R Larson; Daniel Zenklusen; Bin Wu; Jeffrey A Chao; Robert H Singer
Journal: Science Date: 2011-04-22 Impact factor: 47.728

Review 2. Transcription goes digital.

Authors: Timothée Lionnet; Robert H Singer
Journal: EMBO Rep Date: 2012-04-02 Impact factor: 8.807

10. Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos.

Authors: Satoshi H Namekawa; Jeannie T Lee
Journal: Nat Protoc Date: 2011-02-10 Impact factor: 13.491

Imaging transcription in living cells.

1. The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.

2. FRAP reveals that mobility of oestrogen receptor-alpha is ligand- and proteasome-dependent.

Review 3. Kinetic modelling approaches to in vivo imaging.

4. Modulation of RNA polymerase assembly dynamics in transcriptional regulation.

5. Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei.

6. Ligand-specific dynamics of the progesterone receptor in living cells and during chromatin remodeling in vitro.

7. RNA dynamics in live Escherichia coli cells.

8. P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo.

9. Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes.

10. Photobleaching reveals complex effects of inhibitors on transcribing RNA polymerase II in living cells.

1. Real-time observation of transcription initiation and elongation on an endogenous yeast gene.

Review 2. Transcription goes digital.

3. Analytical distribution and tunability of noise in a model of promoter progress.

4. Quantitative Analysis of Intracellular Fluorescent Foci in Live Bacteria.

Review 5. An architectural genetic and epigenetic perspective.

6. Genome-wide measurement of protein-DNA binding dynamics using competition ChIP.

Review 7. Mechanisms of specificity in neuronal activity-regulated gene transcription.

8. Subnuclear segregation of genes and core promoter factors in myogenesis.

9. On the right track: following the nucleo-cytoplasmic path of an mRNA.

10. Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos.