Literature DB >> 19414256

A simple method for monitoring protein-DNA interactions.

Annabelle Fülöp1, Dumitru Arian, Andriy Lysenko, Andriy Mokhir.   

Abstract

A simple, efficient and cheap method is reported for monitoring interactions between single stranded desoxyribonucleic acids and proteins, using fluorescence spectroscopy and complexes of 5'-dye-DNA conjugates with bovine serum albumin as probes. In the presence of a single stranded DNA-binding protein the complexes with bovine serum albumin are disrupted, which results in a reduction of fluorescence intensity.

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Year:  2009        PMID: 19414256     DOI: 10.1016/j.bmcl.2009.03.167

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN: 0960-894X            Impact factor:   2.823


  1 in total

1.  Ethyl 2-[1,3-dioxo-6-(piperidin-1-yl)-2,3-dihydro-1H-benz[de]isoquinolin-2-yl]acetate.

Authors:  Song Xia; Chun-Ling Zheng; Fei-Fei He; Ya-Bin Shi; Hai-Bo Wang
Journal:  Acta Crystallogr Sect E Struct Rep Online       Date:  2011-06-18
  1 in total

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