| Literature DB >> 19407142 |
Sang-Youl Park1, Pauline Fung, Noriyuki Nishimura, Davin R Jensen, Hiroaki Fujii, Yang Zhao, Shelley Lumba, Julia Santiago, Americo Rodrigues, Tsz-Fung F Chow, Simon E Alfred, Dario Bonetta, Ruth Finkelstein, Nicholas J Provart, Darrell Desveaux, Pedro L Rodriguez, Peter McCourt, Jian-Kang Zhu, Julian I Schroeder, Brian F Volkman, Sean R Cutler.
Abstract
Type 2C protein phosphatases (PP2Cs) are vitally involved in abscisic acid (ABA) signaling. Here, we show that a synthetic growth inhibitor called pyrabactin functions as a selective ABA agonist. Pyrabactin acts through PYRABACTIN RESISTANCE 1 (PYR1), the founding member of a family of START proteins called PYR/PYLs, which are necessary for both pyrabactin and ABA signaling in vivo. We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We conclude that PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs. Our results illustrate the power of the chemical genetic approach for sidestepping genetic redundancy.Entities:
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Year: 2009 PMID： 19407142 PMCID： PMC2827199 DOI： 10.1126/science.1173041
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728