Seiichi Yoshida1, Hideaki Takahashi. 1. Department of Neurosurgery, Niigata Cancer Center Hospital, Niigata, Japan. brain@niigata-cc.jp
Abstract
BACKGROUND AND OBJECTIVES: The objectives of this study were to investigate the role of growth factors and matrix metalloproteinases in the brain metastasis. METHODS: Tumors from 40 patients with brain metastasis (30 lung cancer, 10 breast cancer) were studied to investigate the expression-patterns of EGFR, MT1-MMP, MMP7, and CD44 using the immunostaining and RT -PCR analysis. RESULTS: EGFR immunostaining was detected in 92% (23/25) and 70% (7/10) of brain metastasis from lung adenocarcinoma and breast cancer, respectively. MT1-MMP immunostaining was also detected in 80% (20/25) and 50% (5/10) of brain metastasis from lung adenocarcinoma and breast cancer, respectively. EGFR mRNA was detected in 83.3% (25/30) and 70% (7/10) of brain metastasis from lung cancer and breast cancer, respectively. MT1-MMP mRNA was detected in 73.3% (22/30) specimens of brain metastasis from lung cancer, and only 13.3% (4/30) specimens of these patients expressed MMP7 mRNA. CONCLUSIONS: The high expression of EGFR and MT1-MMP indicates that a extracellular matrix remodeling may be playing an important role in brain metastasis. (c) 2009 Wiley-Liss, Inc.
BACKGROUND AND OBJECTIVES: The objectives of this study were to investigate the role of growth factors and matrix metalloproteinases in the brain metastasis. METHODS: Tumors from 40 patients with brain metastasis (30 lung cancer, 10 breast cancer) were studied to investigate the expression-patterns of EGFR, MT1-MMP, MMP7, and CD44 using the immunostaining and RT -PCR analysis. RESULTS:EGFR immunostaining was detected in 92% (23/25) and 70% (7/10) of brain metastasis from lung adenocarcinoma and breast cancer, respectively. MT1-MMP immunostaining was also detected in 80% (20/25) and 50% (5/10) of brain metastasis from lung adenocarcinoma and breast cancer, respectively. EGFR mRNA was detected in 83.3% (25/30) and 70% (7/10) of brain metastasis from lung cancer and breast cancer, respectively. MT1-MMP mRNA was detected in 73.3% (22/30) specimens of brain metastasis from lung cancer, and only 13.3% (4/30) specimens of these patients expressed MMP7 mRNA. CONCLUSIONS: The high expression of EGFR and MT1-MMP indicates that a extracellular matrix remodeling may be playing an important role in brain metastasis. (c) 2009 Wiley-Liss, Inc.
Authors: André Luis Giacometti Conceição; Erica Babeto; Natalia Maria Candido; Fernanda Craveiro Franco; Débora Aparecida Pires de Campos Zuccari; Jane Lopes Bonilha; José Antônio Cordeiro; Marilia Freitas Calmon; Paula Rahal Journal: J Cancer Date: 2015-05-23 Impact factor: 4.207
Authors: David Benito-Alifonso; Barbara Richichi; Veronica Baldoneschi; Monica Berry; Marco Fragai; Gianluca Salerno; M Carmen Galan; Cristina Nativi Journal: ACS Omega Date: 2018-08-23