OBJECTIVES: In regenerative medicine, surface engineering of bioinert synthetic materials is often required in order to introduce bioactive species that can promote cell adhesion, proliferation, viability and enhanced ECM-secretion functions. The aim of this work is to study cell interaction with alumina-modified surfaces. MATERIAL AND METHODS: In this work, chemical properties of alumina surface were changed by a reaction at the surface of alumina with low molecular weight dicarboxylic acid, which produced carboxyl groups. RESULTS: These carboxyl groups were able to complex with Ca2+ on the surface, forming sites of precipitation for calcium phosphates that make alumina biocompatible, as indicated by cell culture of pre-osteoblasts (MC3T3-E1 cell line). CONCLUSIONS: The procedure presented in this work shows that the insertion of specific functional groups on the surface of alumina increases cell interaction with the surface of alumina. This knowledge can be important in oral science and orthopedics, for the construction of prosthesis.
OBJECTIVES: In regenerative medicine, surface engineering of bioinert synthetic materials is often required in order to introduce bioactive species that can promote cell adhesion, proliferation, viability and enhanced ECM-secretion functions. The aim of this work is to study cell interaction with alumina-modified surfaces. MATERIAL AND METHODS: In this work, chemical properties of alumina surface were changed by a reaction at the surface of alumina with low molecular weight dicarboxylic acid, which produced carboxyl groups. RESULTS: These carboxyl groups were able to complex with Ca2+ on the surface, forming sites of precipitation for calcium phosphates that make alumina biocompatible, as indicated by cell culture of pre-osteoblasts (MC3T3-E1 cell line). CONCLUSIONS: The procedure presented in this work shows that the insertion of specific functional groups on the surface of alumina increases cell interaction with the surface of alumina. This knowledge can be important in oral science and orthopedics, for the construction of prosthesis.
Authors: Thais Accorsi-Mendonça; Willian Fernando Zambuzzi; Clóvis Monteiro Bramante; Tânia Mari Cestari; Rumio Taga; Márcia Sader; Glória Dulce de Almeida Soares; José Mauro Granjeiro Journal: J Mater Sci Mater Med Date: 2011-03-20 Impact factor: 3.896
Authors: Willian F Zambuzzi; Estevam A Bonfante; Ryo Jimbo; Mariko Hayashi; Martin Andersson; Gutemberg Alves; Esther R Takamori; Paulo J Beltrão; Paulo G Coelho; José M Granjeiro Journal: PLoS One Date: 2014-07-07 Impact factor: 3.240