Literature DB >> 19394295

SR protein family members display diverse activities in the formation of nascent and mature mRNPs in vivo.

Aparna K Sapra1, Minna-Liisa Ankö, Inna Grishina, Mike Lorenz, Marta Pabis, Ina Poser, Jarod Rollins, Eva-Maria Weiland, Karla M Neugebauer.   

Abstract

The SR proteins are a family of pre-mRNA splicing factors with additional roles in gene regulation. To investigate individual family members in vivo, we generated a comprehensive panel of stable cell lines expressing GFP-tagged SR proteins under endogenous promoter control. Recruitment of SR proteins to nascent FOS RNA was transcription dependent and RNase sensitive, with unique patterns of accumulation along the gene specified by the RNA recognition motifs (RRMs). In addition, all SR protein interactions with Pol II were RNA dependent, indicating that SR proteins are not preassembled with Pol II. SR protein interactions with RNA were confirmed in situ by FRET/FLIM. Interestingly, SC35-GFP also exhibited FRET with DNA and failed to associate with cytoplasmic mRNAs, whereas all other SR proteins underwent nucleocytoplasmic shuttling and associated with specific nuclear and cytoplasmic mRNAs. Because different constellations of SR proteins bound nascent, nuclear, and cytoplasmic mRNAs, mRNP remodeling must occur throughout an mRNA's lifetime.

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Year:  2009        PMID： 19394295     DOI： 10.1016/j.molcel.2009.02.031

Source DB:  PubMed          Journal:  Mol Cell        ISSN： 1097-2765            Impact factor:   17.970


  70 in total

1.  Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles.

Authors:  Ryu Miyagawa; Keiko Tano; Rie Mizuno; Yo Nakamura; Kenichi Ijiri; Randeep Rakwal; Junko Shibato; Yoshinori Masuo; Akila Mayeda; Tetsuro Hirose; Nobuyoshi Akimitsu
Journal:  RNA       Date:  2012-02-21       Impact factor: 4.942

2.  Epstein-Barr Virus SM protein utilizes cellular splicing factor SRp20 to mediate alternative splicing.

Authors:  Dinesh Verma; Swarna Bais; Melusine Gaillard; Sankar Swaminathan
Journal:  J Virol       Date:  2010-09-01       Impact factor: 5.103

Review 3.  The RNAissance family: SR proteins as multifaceted regulators of gene expression.

Authors:  Jonathan M Howard; Jeremy R Sanford
Journal:  Wiley Interdiscip Rev RNA       Date:  2014-08-22       Impact factor: 9.957

4.  Co-transcriptional splicing of constitutive and alternative exons.

Authors:  Amy Pandya-Jones; Douglas L Black
Journal:  RNA       Date:  2009-08-05       Impact factor: 4.942

5.  Rapid-response splicing reporter screens identify differential regulators of constitutive and alternative splicing.

Authors:  Ihab Younis; Michael Berg; Daisuke Kaida; Kimberly Dittmar; Congli Wang; Gideon Dreyfuss
Journal:  Mol Cell Biol       Date:  2010-02-01       Impact factor: 4.272

Review 6.  Nascent RNA and the Coordination of Splicing with Transcription.

Authors:  Karla M Neugebauer
Journal:  Cold Spring Harb Perspect Biol       Date:  2019-08-01       Impact factor: 10.005

7.  Utilizing Yeast Surface Human Proteome Display Libraries to Identify Small Molecule-Protein Interactions.

Authors:  Scott Bidlingmaier; Bin Liu
Journal:  Methods Mol Biol       Date:  2015

Review 8.  How cells get the message: dynamic assembly and function of mRNA-protein complexes.

Authors:  Michaela Müller-McNicoll; Karla M Neugebauer
Journal:  Nat Rev Genet       Date:  2013-03-12       Impact factor: 53.242

Review 9.  Regulation of splicing by SR proteins and SR protein-specific kinases.

Authors:  Zhihong Zhou; Xiang-Dong Fu
Journal:  Chromosoma       Date:  2013-03-24       Impact factor: 4.316

10.  The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.

Authors:  Martina Huranová; Ivan Ivani; Ales Benda; Ina Poser; Yehuda Brody; Martin Hof; Yaron Shav-Tal; Karla M Neugebauer; David Stanek
Journal:  J Cell Biol       Date:  2010-10-04       Impact factor: 10.539
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