Literature DB >> 19394096

Evaluation of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus in sera of domestic ruminants in France.

Catherine Cêtre-Sossah1, Agnès Billecocq, Renaud Lancelot, Cédric Defernez, Jacques Favre, Michèle Bouloy, Dominique Martinez, Emmanuel Albina.   

Abstract

This paper describes the sensitivity and specificity of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus (RVFV) from sera of French domestic ruminants. Field samples were collected from mainland France for the known-negative sera (cattle=191, goats=119, sheep=192) and from ruminants of a French overseas territory (Mayotte) for the known-positive sera. A cut-off value of 43% was determined for all species, achieving a sensitivity and specificity of 100% and a concordance of 100% with the species-specific threshold recommended by the manufacturer. Our results demonstrate that this ELISA may be a suitable diagnostic tool for disease surveillance programmes and import/export veterinary certification of French cattle, goats and sheep.

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Year:  2009        PMID: 19394096     DOI: 10.1016/j.prevetmed.2009.03.011

Source DB:  PubMed          Journal:  Prev Vet Med        ISSN: 0167-5877            Impact factor:   2.670


  4 in total

1.  Evaluation of an Indirect Enzyme-Linked Immunosorbent Assay Based on Recombinant Baculovirus-Expressed Rift Valley Fever Virus Nucleoprotein as the Diagnostic Antigen.

Authors:  Bonto Faburay; William C Wilson; Arss Secka; Barbara Drolet; D Scott McVey; Juergen A Richt
Journal:  J Clin Microbiol       Date:  2019-09-24       Impact factor: 5.948

2.  Rift Valley fever virus(Bunyaviridae: Phlebovirus): an update on pathogenesis, molecular epidemiology, vectors, diagnostics and prevention.

Authors:  Michel Pepin; Michele Bouloy; Brian H Bird; Alan Kemp; Janusz Paweska
Journal:  Vet Res       Date:  2010 Nov-Dec       Impact factor: 3.683

3.  Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants.

Authors:  Janusz T Pawęska; Petrus Jansen van Vuren; Veerle Msimang; Modu Moustapha Lô; Yaya Thiongane; Leopold K Mulumba-Mfumu; Alqadasi Mansoor; José M Fafetine; Joseph W Magona; Hiver Boussini; Barbara Bażanow; William C Wilson; Michel Pepin; Hermann Unger; Gerrit Viljoen
Journal:  Viruses       Date:  2021-08-19       Impact factor: 5.048

4.  First External Quality Assessment of Molecular and Serological Detection of Rift Valley Fever in the Western Mediterranean Region.

Authors:  Federica Monaco; Gian Mario Cosseddu; Baba Doumbia; Hafsa Madani; Fatiha El Mellouli; Miguel Angel Jiménez-Clavero; Soufien Sghaier; Philippe Marianneau; Catherine Cetre-Sossah; Andrea Polci; Sandra Lacote; Magtouf Lakhdar; Jovita Fernandez-Pinero; Chabane Sari Nassim; Chiara Pinoni; Andrea Capobianco Dondona; Carmina Gallardo; Taoufiq Bouzid; Annamaria Conte; Grazia Bortone; Giovanni Savini; Antonio Petrini; Lilian Puech
Journal:  PLoS One       Date:  2015-11-13       Impact factor: 3.240

  4 in total

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