Tsunehisa Ohno1, Shigeru Hirano, Bernard Rousseau. 1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, TN, USA.
Abstract
OBJECTIVES: To measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3 during the inflammatory, proliferative, and remodeling phases of rat vocal fold injury. STUDY DESIGN: Prospective, animal model. SUBJECTS AND METHODS: Vocal folds were injured in 30 rats. Injured specimens were harvested on postinjury days 1, 3, 7, 14, 28, and 56. Five uninjured rats were used for polymerase chain reaction (PCR) control. Real-time PCR was used to measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3. RESULTS: Compared with control, expression of procollagen-I and -III were significantly decreased on postinjury days 1 and 56; decorin expression was significantly decreased on postinjury days 1, 3, 7, and 56; HAS-1 expression was significantly decreased on postinjury days 3, 7, 14, 28, and 56; HAS-2 expression was significantly decreased on postinjury days 28 and 56; HAS-3 expression was significantly decreased on postinjury day 56. CONCLUSIONS: Results revealed time-dependent alterations in the expression of genes coding procollagen-I and -III, decorin, and HAS-1, -2, and -3. Knowledge of the temporal regulation of these genes and underlying histology will be used in future studies to investigate molecular approaches for manipulation of vocal fold injury.
OBJECTIVES: To measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3 during the inflammatory, proliferative, and remodeling phases of rat vocal fold injury. STUDY DESIGN: Prospective, animal model. SUBJECTS AND METHODS: Vocal folds were injured in 30 rats. Injured specimens were harvested on postinjury days 1, 3, 7, 14, 28, and 56. Five uninjured rats were used for polymerase chain reaction (PCR) control. Real-time PCR was used to measure the expression of procollagen-I and -III, decorin, and hyaluronan synthase (HAS)-1, -2, and -3. RESULTS: Compared with control, expression of procollagen-I and -III were significantly decreased on postinjury days 1 and 56; decorin expression was significantly decreased on postinjury days 1, 3, 7, and 56; HAS-1 expression was significantly decreased on postinjury days 3, 7, 14, 28, and 56; HAS-2 expression was significantly decreased on postinjury days 28 and 56; HAS-3 expression was significantly decreased on postinjury day 56. CONCLUSIONS: Results revealed time-dependent alterations in the expression of genes coding procollagen-I and -III, decorin, and HAS-1, -2, and -3. Knowledge of the temporal regulation of these genes and underlying histology will be used in future studies to investigate molecular approaches for manipulation of vocal fold injury.
Authors: Erik R Swanson; Tsunehisa Ohno; Dave Abdollahian; C Gaelyn Garrett; Bernard Rousseau Journal: Otolaryngol Head Neck Surg Date: 2010-10 Impact factor: 3.497
Authors: Atsushi Suehiro; Jonathan M Bock; Joseph E Hall; C Gaelyn Garrett; Bernard Rousseau Journal: Laryngoscope Date: 2012-01-17 Impact factor: 3.325