| Literature DB >> 19383295 |
P A Usher1, A M Sieuwerts, A Bartels, U Lademann, H J Nielsen, L Holten-Andersen, J A Foekens, N Brünner, H Offenberg.
Abstract
TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients. The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.Entities:
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Year: 2007 PMID： 19383295 PMCID： PMC5543896 DOI： 10.1016/j.molonc.2007.05.002
Source DB: PubMed Journal: Mol Oncol ISSN： 1574-7891 Impact factor: 6.603