| Literature DB >> 19383245 |
X-D Zhou1, X-Y Wang, F-J Qu, Y-H Zhong, X-D Lu, P Zhao, D-H Wang, Q-B Huang, L Zhang, X-G Li.
Abstract
Various malignant cancers have been found to contain a sub-population of stem cell-like tumour cells, or cancer stem cells (CSCs), however, culture methods for CSCs and the size of the fraction of CSCs in C6, which is a commonly used glioma cell line, remain controversial. In this study, we demonstrated that the C6 cell line contains a fraction of tumour cells that can form tumour spheres in a simplified serum-free neural stem cell medium and express CD133 and nestin, which are widely-used markers for brain CSCs. Immunohistochemistry and immunofluorescence confirmed the existence of CSCs both in the C6 cell line and C6 xenografts. Flow cytometry demonstrated that 4.02% of cells in the C6 cell line and 4.21% in the C6 xenografts presented as CSCs. These results confirm the fraction of CSCs in the C6 cell line and provide a simple and effective method for isolation of CSCs to study the initiation and progression of human glioma and, possibly, other malignant tumours.Entities:
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Year: 2009 PMID： 19383245 DOI： 10.1177/147323000903700226
Source DB: PubMed Journal: J Int Med Res ISSN： 0300-0605 Impact factor: 1.671