Literature DB >> 19382578

Refinement and extension of AOAC Method 2005.06 to include additional toxins in mussels: single-laboratory validation.

Andrew D Turner1, Deirdre M Norton, Robert G Hatfield, Steven Morris, Allan R Reese, Myriam Algoet, David N Lees.   

Abstract

A single-laboratory validation study was undertaken for the analysis of paralytic shellfish poisoning (PSP) toxins in common mussels, extending AOAC Official Method 2005.06 to include the additional toxins dcNEO and dcGTX2,3. The method was refined to improve toxin oxidation product stability, analytical sensitivity of N-hydroxylated toxins, and throughput. Validation was performed to characterize the method for selectivity, sensitivity, linearity, precision, repeatability, recovery, ruggedness, and uncertainty. Parallel testing of naturally contaminated mussels enabled comparison of sample toxicities obtained using mouse bioassay (MBA) and high-performance liquid chromatographic (HPLC) methodologies. Performance characteristics of the method are reported for all commercially available certified reference toxins. Results from the MBA and HPLC methods were well correlated, and the analytical method has been instigated as the sole monitoring tool for UK official control surveillance of PSP toxins in common mussels.

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Year:  2009        PMID： 19382578

Source DB:  PubMed          Journal:  J AOAC Int        ISSN： 1060-3271            Impact factor:   1.913


  10 in total

1.  Highly toxic Microcystis aeruginosa strain, isolated from São Paulo-Brazil, produce hepatotoxins and paralytic shellfish poison neurotoxins.

Authors:  Célia L Sant'Anna; Luciana R de Carvalho; Marli F Fiore; Maria Estela Silva-Stenico; Adriana S Lorenzi; Fernanda R Rios; Katsuhiro Konno; Carlos Garcia; Nestor Lagos
Journal:  Neurotox Res       Date:  2010-04-08       Impact factor: 3.911

2.  Studies in the use of magnetic microspheres for immunoaffinity extraction of paralytic shellfish poisoning toxins from shellfish.

Authors:  Raymond Devlin; Katrina Campbell; Kentaro Kawatsu; Christopher Elliott
Journal:  Toxins (Basel)       Date:  2011-01-04       Impact factor: 4.546

3.  Influence of different shellfish matrices on the separation of PSP toxins using a postcolumn oxidation liquid chromatography method.

Authors:  Verónica Rey; Amparo Alfonso; Luis M Botana; Ana M Botana
Journal:  Toxins (Basel)       Date:  2015-04-15       Impact factor: 4.546

4.  Fatal Canine Intoxications Linked to the Presence of Saxitoxins in Stranded Marine Organisms Following Winter Storm Activity.

Authors:  Andrew D Turner; Monika Dhanji-Rapkova; Karl Dean; Steven Milligan; Mike Hamilton; Julie Thomas; Chris Poole; Jo Haycock; Jo Spelman-Marriott; Alice Watson; Katherine Hughes; Bridget Marr; Alan Dixon; Lewis Coates
Journal:  Toxins (Basel)       Date:  2018-02-26       Impact factor: 4.546

5.  The Application of Nanopore Sequencing Technology to the Study of Dinoflagellates: A Proof of Concept Study for Rapid Sequence-Based Discrimination of Potentially Harmful Algae.

Authors:  Robert G Hatfield; Frederico M Batista; Timothy P Bean; Vera G Fonseca; Andres Santos; Andrew D Turner; Adam Lewis; Karl J Dean; Jaime Martinez-Urtaza
Journal:  Front Microbiol       Date:  2020-05-08       Impact factor: 5.640

6.  Development of a TaqMan qPCR assay for detection of Alexandrium spp and application to harmful algal bloom monitoring.

Authors:  Robert G Hatfield; Timothy Bean; Andrew D Turner; David N Lees; James Lowther; Adam Lewis; Craig Baker-Austin
Journal:  Toxicon X       Date:  2019-03-08

7.  What caused the UK's largest common dolphin (Delphinus delphis) mass stranding event?

Authors:  Paul D Jepson; Robert Deaville; Karina Acevedo-Whitehouse; James Barnett; Andrew Brownlow; Robert L Brownell; Frances C Clare; Nick Davison; Robin J Law; Jan Loveridge; Shaheed K Macgregor; Steven Morris; Sinéad Murphy; Rod Penrose; Matthew W Perkins; Eunice Pinn; Henrike Seibel; Ursula Siebert; Eva Sierra; Victor Simpson; Mark L Tasker; Nick Tregenza; Andrew A Cunningham; Antonio Fernández
Journal:  PLoS One       Date:  2013-04-30       Impact factor: 3.240

8.  Results of a Saxitoxin Proficiency Test Including Characterization of Reference Material and Stability Studies.

Authors:  Kirsi Harju; Marja-Leena Rapinoja; Marc-André Avondet; Werner Arnold; Martin Schär; Werner Luginbühl; Anke Kremp; Sanna Suikkanen; Harri Kankaanpää; Stephen Burrell; Martin Söderström; Paula Vanninen
Journal:  Toxins (Basel)       Date:  2015-11-25       Impact factor: 4.546

9.  Detection of Tetrodotoxin Shellfish Poisoning (TSP) Toxins and Causative Factors in Bivalve Molluscs from the UK.

Authors:  Andrew D Turner; Monika Dhanji-Rapkova; Lewis Coates; Lesley Bickerstaff; Steve Milligan; Alison O'Neill; Dermot Faulkner; Hugh McEneny; Craig Baker-Austin; David N Lees; Myriam Algoet
Journal:  Mar Drugs       Date:  2017-08-30       Impact factor: 5.118

10.  Revisiting the HPLC-FLD Method to Quantify Paralytic Shellfish Toxins: C3,4 Quantification and the First Steps towards Validation.

Authors:  Joana F Leal; Maria L S Cristiano
Journal:  Toxins (Basel)       Date:  2022-02-27       Impact factor: 4.546
  10 in total

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