| Literature DB >> 19381975 |
Richard Redon1, Diane Rigler, Nigel P Carter.
Abstract
The spatial resolution of microarray-based comparative genomic hybridization (array-CGH) is dependent on the length and density of target DNA sequences covering the chromosomal region of interest. Here we describe the methods developed at the Wellcome Trust Sanger Institute (Cambridge, UK) to construct microarrays comprising large-insert clones available through genome sequencing projects. These methods are applicable to Bacterial and Phage Artificial Chromosomes (BAC and PAC) as well as fosmid and cosmid clones. The protocols are scalable for the construction of microarrays composed of several hundreds up to several ten thousands clones.Entities:
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Year: 2009 PMID： 19381975 PMCID： PMC2867217 DOI： 10.1007/978-1-59745-538-1_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745