Literature DB >> 17126854

A fast and efficient procedure to produce scFvs specific for large macromolecular complexes.

Si Wu1, Ailong Ke, Jennifer A Doudna.   

Abstract

We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.

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Year:  2006        PMID： 17126854      PMCID： PMC2920608          DOI： 10.1016/j.jim.2006.10.005

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  20 in total

1.  Use of phage display and high-density screening for the isolation of an antibody against the 51-kDa subunit of complex I.

Authors:  John L Rubinstein; Lucy J Holt; John E Walker; Ian M Tomlinson
Journal:  Anal Biochem       Date:  2003-03-15       Impact factor: 3.365

2.  Generation of affinity matured scFv antibodies against mouse neural cell adhesion molecule L1 by phage display.

Authors:  Ling Dong; Suzhen Chen; Udo Bartsch; Melitta Schachner
Journal:  Biochem Biophys Res Commun       Date:  2003-01-31       Impact factor: 3.575

3.  Single-chain variable fragment (scFv) antibodies against rotavirus NSP4 enterotoxin generated by phage display.

Authors:  Jesús Rodríguez-Díaz; Vicente Monedero; Gaspar Pérez-Martínez; Javier Buesa
Journal:  J Virol Methods       Date:  2004-11       Impact factor: 2.014

4.  Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle.

Authors:  H D Bernstein; M A Poritz; K Strub; P J Hoben; S Brenner; P Walter
Journal:  Nature       Date:  1989-08-10       Impact factor: 49.962

5.  Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains.

Authors:  K Römisch; J Webb; J Herz; S Prehn; R Frank; M Vingron; B Dobberstein
Journal:  Nature       Date:  1989-08-10       Impact factor: 49.962

6.  An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle.

Authors:  M A Poritz; H D Bernstein; K Strub; D Zopf; H Wilhelm; P Walter
Journal:  Science       Date:  1990-11-23       Impact factor: 47.728

7.  Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor.

Authors:  J D Miller; H D Bernstein; P Walter
Journal:  Nature       Date:  1994-02-17       Impact factor: 49.962

8.  Generation and characterization of a novel single-chain antibody fragment specific against human fibrin clots from phage display antibody library.

Authors:  Jun Peng Yan; Ju Ho Ko; Yi Peng Qi
Journal:  Thromb Res       Date:  2004       Impact factor: 3.944

9.  Phage display of ScFv peptides recognizing the thymidine(6-4)thymidine photoproduct.

Authors:  A G Zavala; T Lancaster; J D Groopman; P T Strickland; S Chandrasegaran
Journal:  Nucleic Acids Res       Date:  2000-04-01       Impact factor: 16.971

10.  Isolation and characterisation of vascular endothelial growth factor-165 specific scFv fragments by phage display.

Authors:  K A Smith; N Kirkpatrick; L A Madden; K P Topping; J R T Monson; J Greenman
Journal:  Int J Oncol       Date:  2003-02       Impact factor: 5.650
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  6 in total

1.  SRP RNA provides the physiologically essential GTPase activation function in cotranslational protein targeting.

Authors:  Fai Y Siu; Richard J Spanggord; Jennifer A Doudna
Journal:  RNA       Date:  2006-12-12       Impact factor: 4.942

2.  Improvement of the crystallizability and expression of an RNA crystallization chaperone.

Authors:  Priyadarshini P Ravindran; Annie Héroux; Jing-Dong Ye
Journal:  J Biochem       Date:  2011-07-23       Impact factor: 3.387

3.  Isolation of scFv fragments specific for monokine induced by interferon-gamma (MIG) using phage display.

Authors:  Edward Eteshola
Journal:  J Immunol Methods       Date:  2010-04-09       Impact factor: 2.303

4.  The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae.

Authors:  D Lobová; A Cízek; V Celer
Journal:  Folia Microbiol (Praha)       Date:  2009-04-18       Impact factor: 2.099

5.  Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies.

Authors:  Theresa Weber; Sibylle Pscherer; Ulrike Gamerdinger; Andrea Teigler-Schlegel; Natalja Rutz; Wolfgang Blau; Mathias Rummel; Stefan Gattenlöhner; Mehmet Kemal Tur
Journal:  Mol Med Rep       Date:  2021-09-07       Impact factor: 2.952

6.  A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick.

Authors:  Sonu Gandhi; Ivneet Banga; Pawan Kumar Maurya; Sergei A Eremin
Journal:  RSC Adv       Date:  2018-01-04       Impact factor: 4.036
  6 in total

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