Literature DB >> 19378040

Introduction to protein blotting.

Biji T Kurien1, R Hal Scofield.   

Abstract

Protein blotting is a powerful and important procedure for the immunodetection of proteins following electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer.

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Year:  2009        PMID: 19378040     DOI: 10.1007/978-1-59745-542-8_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  7 in total

1.  Problems with multiple use of transfer buffer in protein electrophoretic transfer.

Authors:  Yaser Dorri; Biji T Kurien; R Hal Scofield
Journal:  J Biomol Tech       Date:  2010-04

2.  A protein multiplex microarray substrate with high sensitivity and specificity.

Authors:  Dolores A Fici; William McCormick; David W Brown; John E Herrmann; Vikram Kumar; Zuheir L Awdeh
Journal:  J Immunol Methods       Date:  2010-10-23       Impact factor: 2.303

Review 3.  Considerations when quantitating protein abundance by immunoblot.

Authors:  Alicia A McDonough; Luciana C Veiras; Jacqueline N Minas; Donna Lee Ralph
Journal:  Am J Physiol Cell Physiol       Date:  2014-12-24       Impact factor: 4.249

4.  A novel method that improves sensitivity of protein detection in PAGE and Western blot.

Authors:  Ainara Vallejo-Illarramendi; Denise K Marciano; Louis F Reichardt
Journal:  Electrophoresis       Date:  2013-03-15       Impact factor: 3.535

5.  Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds.

Authors:  Nausika Betriu; Lourdes Recha-Sancho; Carlos E Semino
Journal:  J Vis Exp       Date:  2018-06-13       Impact factor: 1.355

6.  Comparison on virulence and immunogenicity of two recombinant vaccinia vaccines, Tian Tan and Guang9 strains, expressing the HIV-1 envelope gene.

Authors:  Rong Zhu; Weijin Huang; Wenbo Wang; Qiang Liu; Jianhui Nie; Shufang Meng; Yongxin Yu; Youchun Wang
Journal:  PLoS One       Date:  2012-11-06       Impact factor: 3.240

7.  Alleviative effects of total flavones of Glycyrrhiza uralensis Fisch on oxidative stress and lipid metabolism disorder induced by high-fat diet in intestines of Tilapia (Oreochromis niloticus).

Authors:  Jinliang Du; Li-Ping Cao; Rui Jia; Zhengyan Gu; Qin He; Pao Xu; Guojun Yin; Yuzhong Ma
Journal:  3 Biotech       Date:  2021-06-20       Impact factor: 2.893

  7 in total

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